{"product_id":"ptcd1-antibody-pentatricopeptide-repeat-domain-containing-protein-1-bha17135603","title":"PTCD1 Antibody \/ Pentatricopeptide repeat domain-containing protein 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePTCD1 Antibody \/ Pentatricopeptide repeat domain-containing protein 1 is a anti-PTCD1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Mitochondria.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PTCD1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePTCD1 antibody detects Pentatricopeptide repeat domain-containing protein 1, a mitochondrial RNA-binding protein that regulates mitochondrial gene expression and RNA metabolism. Encoded by the PTCD1 gene on chromosome 2q31.3, this protein contains multiple tandem pentatricopeptide repeat (PPR) motifs that mediate specific binding to mitochondrial RNA transcripts. PTCD1 functions as a post-transcriptional regulator that controls the stability, processing, and translation of mitochondrial mRNAs essential for oxidative phosphorylation. By coordinating RNA maturation and ribosome assembly within mitochondria, PTCD1 ensures proper energy production and cellular respiration.\u003cbr\u003e\u003cbr\u003ePTCD1 belongs to a large family of PPR proteins that are characterized by their modular RNA-binding repeats. In mammalian cells, PTCD1 localizes to the mitochondrial matrix and associates with mitochondrial ribonucleoprotein complexes. Functional studies indicate that PTCD1 modulates expression of key mitochondrial-encoded subunits of the respiratory chain, including components of complexes I and IV. Knockdown or loss of PTCD1 disrupts mitochondrial translation, leading to impaired ATP synthesis and increased reactive oxygen species production. As such, PTCD1 plays a central role in maintaining mitochondrial integrity and energy homeostasis.\u003cbr\u003e\u003cbr\u003eThe PTCD1 antibody is widely used in studies of mitochondrial biology, RNA processing, and metabolic regulation. In western blot assays, PTCD1 is detected as a ~75 kilodalton band, while immunofluorescence reveals punctate staining consistent with mitochondrial localization. Expression profiling has shown that PTCD1 is abundant in metabolically active tissues such as heart, skeletal muscle, and brain, where mitochondrial activity is high. The antibody is an essential reagent for examining mitochondrial gene expression pathways and for identifying alterations in mitochondrial function associated with metabolic disorders and neurodegeneration.\u003cbr\u003e\u003cbr\u003eBeyond its core function in RNA metabolism, PTCD1 has been linked to mitochondrial-nuclear communication and apoptosis regulation. It may influence the transcription of nuclear-encoded mitochondrial genes by modulating signaling pathways that respond to mitochondrial dysfunction. Genetic variants of PTCD1 have been associated with susceptibility to metabolic diseases, including type 2 diabetes and mitochondrial encephalomyopathy. The PTCD1 antibody thus enables researchers to explore these regulatory networks and to define the molecular mechanisms that couple RNA metabolism with mitochondrial biogenesis.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PTCD1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PTCD1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PTCD1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PTCD1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PTCD1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047296754029,"sku":"FY12700","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_eadde127-2611-4024-b8c9-e04f007b784b.jpg?v=1782237050","url":"https:\/\/www.ebiohippo.com\/products\/ptcd1-antibody-pentatricopeptide-repeat-domain-containing-protein-1-bha17135603","provider":"BioHippo","version":"1.0","type":"link"}