{"product_id":"pten-antibody-phosphatase-and-tensin-bha17135272","title":"PTEN Antibody \/ Phosphatase and tensin","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePTEN Antibody \/ Phosphatase and tensin is a anti-PTEN Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PTEN\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eThe PTEN antibody targets Phosphatase and tensin homolog, a dual-specificity phosphatase encoded by the PTEN gene. This enzyme is one of the most frequently mutated tumor suppressors in human cancer and plays a crucial role in regulating cell growth, survival, and metabolism. Phosphatase and tensin homolog functions primarily as a lipid phosphatase, dephosphorylating phosphatidylinositol (3,4,5)-trisphosphate (PIP3) to phosphatidylinositol (4,5)-bisphosphate (PIP2), thereby antagonizing the PI3K-AKT signaling pathway. The PTEN antibody enables detection of this critical regulatory protein and supports research into tumor suppression, cell signaling, and metabolic control.\u003cbr\u003e\u003cbr\u003ePhosphatase and tensin homolog acts at the plasma membrane to regulate signal transduction and control downstream kinases that drive proliferation and survival. Loss of PTEN function results in constitutive activation of AKT and mTOR pathways, leading to uncontrolled cell growth and oncogenesis. The PTEN antibody allows visualization of protein expression across tissues, facilitating analysis of its localization in both cytoplasmic and nuclear compartments. Nuclear PTEN has additional roles in maintaining chromosomal stability and DNA repair, expanding its tumor-suppressive functions beyond lipid dephosphorylation.\u003cbr\u003e\u003cbr\u003eMutations or deletions in the PTEN gene occur in many cancers, including glioblastoma, prostate, breast, and endometrial carcinomas. Germline PTEN mutations cause PTEN hamartoma tumor syndromes such as Cowden syndrome, characterized by benign and malignant tumor development. The PTEN antibody is widely used in diagnostic and research settings to assess expression loss and mutation effects in tumor samples. Detection of reduced PTEN levels by immunohistochemistry is an important indicator of dysregulated PI3K signaling and poor prognosis.\u003cbr\u003e\u003cbr\u003eIn addition to tumor suppression, Phosphatase and tensin homolog regulates cellular processes such as migration, apoptosis, and metabolism. It influences insulin signaling, oxidative stress response, and stem cell renewal. The PTEN antibody supports mechanistic studies into these diverse biological roles and enables quantification of protein abundance in cultured cells and tissue models. By revealing PTEN localization and activation states, this antibody provides insight into how phosphorylation, ubiquitination, or oxidation modulate its function under physiological and stress conditions.\u003cbr\u003e\u003cbr\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PTEN - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PTEN - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PTEN - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PTEN - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PTEN+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047286235501,"sku":"FY12369","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_b95100af-0381-49da-acbf-22df5dd0ab9f.jpg?v=1782236989","url":"https:\/\/www.ebiohippo.com\/products\/pten-antibody-phosphatase-and-tensin-bha17135272","provider":"BioHippo","version":"1.0","type":"link"}