{"product_id":"ptger3-antibody-prostaglandin-e2-receptor-ep3-bha17136284","title":"PTGER3 Antibody \/ Prostaglandin E2 receptor EP3","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePTGER3 Antibody \/ Prostaglandin E2 receptor EP3 is a anti-PTGER3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PTGER3\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePTGER3 antibody detects Prostaglandin E2 receptor EP3 subtype, a G protein-coupled receptor (GPCR) encoded by the PTGER3 gene located on chromosome 1p31.1. PTGER3 mediates the biological effects of prostaglandin E2 (PGE2), a key lipid signaling molecule involved in inflammation, pain perception, smooth muscle contraction, and thermoregulation. The EP3 receptor is unique among the four PGE2 receptor subtypes (EP1-EP4) because of its multiple isoforms generated through alternative splicing, each coupling to different G proteins (Gi, Gs, or Gq) and eliciting diverse downstream responses.\u003cbr\u003e\u003cbr\u003eStructurally, PTGER3 is a seven-transmembrane GPCR with extracellular ligand-binding domains and intracellular loops that interact with heterotrimeric G proteins. It belongs to the rhodopsin-like GPCR family, sharing sequence homology with other prostanoid receptors. Co-localization studies show PTGER3 localized on the plasma membrane of smooth muscle cells, immune cells, and neurons. The receptor�s cytoplasmic tail contains regions that determine isoform-specific signaling and regulatory properties.\u003cbr\u003e\u003cbr\u003eFunctionally, PTGER3 mediates PGE2-dependent inhibition of adenylate cyclase via Gi coupling, leading to decreased intracellular cAMP levels. In smooth muscle, this signaling pathway promotes contraction, contributing to uterine and gastrointestinal motility. In the central nervous system, PTGER3 influences fever generation and pain modulation through hypothalamic pathways. The receptor also regulates renal sodium excretion, vascular tone, and platelet aggregation. Known ligands include PGE2 and selective agonists such as sulprostone and misoprostol.\u003cbr\u003e\u003cbr\u003ePTGER3 expression is widespread but particularly high in uterus, kidney, stomach, and brain. Dysregulation of PTGER3 has been associated with inflammatory diseases, reproductive disorders, and cancer. Overexpression contributes to tumor progression by promoting angiogenesis and immune evasion, while inhibition reduces inflammation and hyperalgesia. Pathway associations include prostaglandin signaling, GPCR-mediated calcium signaling, and cAMP regulation. During development, PTGER3 contributes to uterine contractility and thermoregulatory responses.\u003cbr\u003e\u003cbr\u003eThe PTGER3 antibody from\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PTGER3 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PTGER3 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PTGER3 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PTGER3 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PTGER3+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047324115309,"sku":"FY13382","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_1ed2db83-101b-4338-b92b-5e09f23b11b5.jpg?v=1782237114","url":"https:\/\/www.ebiohippo.com\/products\/ptger3-antibody-prostaglandin-e2-receptor-ep3-bha17136284","provider":"BioHippo","version":"1.0","type":"link"}