{"product_id":"ptprn-antibody-receptor-type-tyrosine-protein-phosphatase-like-n-bha17135268","title":"PTPRN Antibody \/ Receptor-type tyrosine-protein phosphatase-like N","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePTPRN Antibody \/ Receptor-type tyrosine-protein phosphatase-like N is a anti-PTPRN Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PTPRN\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eThe PTPRN antibody targets Receptor-type tyrosine-protein phosphatase-like N, a membrane-associated protein encoded by the PTPRN gene. This protein is primarily expressed in neuroendocrine tissues, including pancreatic islets and the brain, where it plays critical roles in vesicle trafficking, hormone secretion, and cell signaling. Receptor-type tyrosine-protein phosphatase-like N is a member of the protein tyrosine phosphatase (PTP) family, but unlike classical PTPs, it lacks catalytic activity due to substitutions within its phosphatase domain. The PTPRN antibody provides researchers with a reliable reagent to study this important secretory granule-associated protein and its involvement in neuroendocrine regulation.\u003cbr\u003e\u003cbr\u003eReceptor-type tyrosine-protein phosphatase-like N, also known as islet antigen 2 (IA-2) and Islet cell antigen 512 (ICA 512), localizes to dense-core secretory vesicles in pancreatic beta cells and neurons. It participates in the exocytosis of insulin, neurotransmitters, and other hormones by regulating vesicle priming and trafficking. The PTPRN antibody enables visualization of IA-2 distribution within endocrine tissues, supporting studies of insulin granule dynamics and secretory mechanisms. Given its expression pattern, PTPRN has become a key marker for endocrine differentiation and synaptic function.\u003cbr\u003e\u003cbr\u003eAutoantibodies against PTPRN are major biomarkers of type 1 diabetes mellitus, detected in pre-symptomatic and newly diagnosed patients. These autoantibodies target epitopes on the cytoplasmic domain of the protein, reflecting autoimmune destruction of pancreatic beta cells. The PTPRN antibody supports research aimed at understanding autoantigen formation and immune recognition mechanisms in diabetes. Its detection of Receptor-type tyrosine-protein phosphatase-like N allows exploration of expression changes in pancreatic tissue and model systems that mimic beta-cell stress or immune attack.\u003cbr\u003e\u003cbr\u003eIn the nervous system, PTPRN modulates synaptic vesicle turnover and neurotransmitter release. It interacts with SNARE proteins and other exocytic machinery components to regulate calcium-dependent secretion. The PTPRN antibody is an essential reagent for characterizing these interactions and mapping protein distribution in brain regions involved in neurotransmission and neuroendocrine signaling. Alterations in IA-2 expression have also been associated with neurodegenerative and psychiatric conditions, suggesting broader roles in neural physiology.\u003cbr\u003e\u003cbr\u003eApplications for the PTPRN antibody include western blotting, immunofluorescence, and immunohistochemistry. These techniques reveal its enrichment in pancreatic islets, pituitary cells, and neuronal terminals.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PTPRN - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PTPRN - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PTPRN - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PTPRN - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PTPRN+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047287611757,"sku":"FY12365","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_3ab87d2e-f185-4613-abc8-86c703ac863a.jpg?v=1782237010","url":"https:\/\/www.ebiohippo.com\/products\/ptprn-antibody-receptor-type-tyrosine-protein-phosphatase-like-n-bha17135268","provider":"BioHippo","version":"1.0","type":"link"}