{"product_id":"ptrhd1-antibody-peptidyl-trna-hydrolase-domain-containing-protein-1-bha17136153","title":"PTRHD1 Antibody \/ Peptidyl-tRNA hydrolase domain-containing protein 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePTRHD1 Antibody \/ Peptidyl-tRNA hydrolase domain-containing protein 1 is a anti-PTRHD1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nucleus, Miochondria.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PTRHD1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC, IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePTRHD1 antibody detects Peptidyl-tRNA hydrolase domain-containing protein 1, a cytoplasmic enzyme-like protein implicated in tRNA metabolism, proteostasis, and neurological function. The UniProt recommended name is Peptidyl-tRNA hydrolase domain-containing protein 1 (PTRHD1). Although it contains a conserved hydrolase-like domain, PTRHD1 may function primarily as a regulator or adaptor rather than a catalytic enzyme, linking translation quality control with neuronal integrity.\u003cbr\u003e\u003cbr\u003eFunctionally, PTRHD1 antibody identifies a 220-amino-acid protein that associates with translational complexes and ubiquitin-proteasome pathways. PTRHD1 is proposed to participate in the recycling of prematurely released peptidyl-tRNAs during protein synthesis, preventing accumulation of stalled translation intermediates. It may also regulate proteasomal activity by binding to ubiquitin-like proteins and adaptors such as UBQLN4, thereby influencing protein degradation and quality control under cellular stress conditions.\u003cbr\u003e\u003cbr\u003eThe PTRHD1 gene is located on chromosome 2p23.3 and is expressed predominantly in brain, testis, and liver. Its expression pattern and domain composition suggest a dual role in neuronal homeostasis and proteostatic regulation. PTRHD1 may serve as a molecular bridge connecting translation fidelity to protein turnover and synaptic function.\u003cbr\u003e\u003cbr\u003ePathologically, biallelic loss-of-function mutations in PTRHD1 cause autosomal recessive early-onset parkinsonism with intellectual disability, highlighting its importance in maintaining neuronal health. Impaired PTRHD1 function results in accumulation of defective translation products and increased cellular stress, leading to neurodegeneration. Research using PTRHD1 antibody supports studies in translational control, proteostasis, and neurogenetic disorders.\u003cbr\u003e\u003cbr\u003ePTRHD1 antibody is validated for western blotting, immunohistochemistry, and immunofluorescence to detect small cytoplasmic regulatory proteins. \u003cbr\u003e\u003cbr\u003eStructurally, Peptidyl-tRNA hydrolase domain-containing protein 1 contains an N-terminal hydrolase-like domain similar to bacterial PTH enzymes but lacking key catalytic residues, suggesting a non-enzymatic regulatory role. The protein's C-terminal region supports interactions with ubiquitin-binding proteins. This antibody facilitates exploration of PTRHD1's role in neuronal proteostasis and translation fidelity.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PTRHD1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PTRHD1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PTRHD1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PTRHD1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PTRHD1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047320183149,"sku":"FY13251","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_21a80507-0648-4fce-8ab9-307f7d027a5f.jpg?v=1782237079","url":"https:\/\/www.ebiohippo.com\/products\/ptrhd1-antibody-peptidyl-trna-hydrolase-domain-containing-protein-1-bha17136153","provider":"BioHippo","version":"1.0","type":"link"}