{"product_id":"puf60-antibody-poly-u-binding-splicing-factor-60-kda-bha17136065","title":"PUF60 Antibody \/ Poly(U)-binding splicing factor 60 kDa","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePUF60 Antibody \/ Poly(U)-binding splicing factor 60 kDa is a anti-PUF60 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PUF60\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, IP, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePUF60 antibody detects Poly(U)-binding splicing factor 60 kDa, an RNA-binding protein that regulates pre-mRNA splicing and transcriptional control. The UniProt recommended name is Poly(U)-binding splicing factor 60 kDa (PUF60). This multifunctional protein binds uridine-rich RNA sequences and facilitates spliceosome assembly by promoting U2 snRNP recruitment to introns.\u003cbr\u003e\u003cbr\u003eFunctionally, PUF60 antibody identifies a 548-amino-acid protein containing two RNA recognition motifs (RRMs) that mediate specific binding to pre-mRNA and single-stranded DNA. PUF60 enhances exon definition and alternative splicing by interacting with U2AF65, SF3B1, and other splicing factors. It also cooperates with transcriptional regulators to coordinate RNA processing and gene expression.\u003cbr\u003e\u003cbr\u003eThe PUF60 gene is located on chromosome 8q24.3 and is ubiquitously expressed, with high levels in brain, testis, and proliferative tissues. PUF60 acts as a bridge between transcription and RNA processing machinery, ensuring accurate and efficient mRNA maturation.\u003cbr\u003e\u003cbr\u003ePathologically, mutations or haploinsufficiency of PUF60 cause developmental syndromes characterized by intellectual disability, short stature, and craniofacial abnormalities. Abnormal PUF60 expression has also been associated with cancer and splicing-related disorders. Research using PUF60 antibody supports studies in RNA metabolism, splicing regulation, and genetic disease mechanisms.\u003cbr\u003e\u003cbr\u003ePUF60 antibody is validated for western blotting, immunoprecipitation, and immunofluorescence to detect splicing factors and RNA-binding proteins. \u003cbr\u003e\u003cbr\u003eStructurally, Poly(U)-binding splicing factor 60 kDa contains tandem RRMs and a glycine-rich C-terminal region that facilitate RNA recognition and spliceosome assembly. This antibody enables exploration of PUF60's role in RNA splicing and gene expression control.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PUF60 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PUF60 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PUF60 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PUF60 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PUF60+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047314186605,"sku":"FY13163","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_481f9e4d-bc69-488a-976d-9a004509f969.jpg?v=1782237100","url":"https:\/\/www.ebiohippo.com\/products\/puf60-antibody-poly-u-binding-splicing-factor-60-kda-bha17136065","provider":"BioHippo","version":"1.0","type":"link"}