{"product_id":"pum3-antibody-pumilio-3-bha17135663","title":"PUM3 Antibody \/ Pumilio 3","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePUM3 Antibody \/ Pumilio 3 is a anti-PUM3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PUM3\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePUM3 antibody detects Pumilio homolog 3 (also known as Pumilio domain-containing protein 3), an RNA-binding protein that participates in post-transcriptional gene regulation and ribosome biogenesis. Encoded by the PUM3 gene on chromosome 9q34.11, this protein is a member of the PUF (Pumilio and FBF) family characterized by their RNA recognition repeats that bind specific sequences in target transcripts. Unlike classical cytoplasmic Pumilio proteins (PUM1 and PUM2), PUM3 is localized to the nucleus and nucleolus, where it regulates pre-rRNA processing and ribosome maturation.\u003cbr\u003e\u003cbr\u003ePUM3 contains eight PUF RNA-binding domains arranged in a crescent-shaped architecture that recognizes conserved 5'-UGUANAUA-3' motifs in RNA targets. It binds ribosomal RNA precursors and ribosome assembly factors, influencing the processing of 28S and 5.8S rRNA. Depletion of PUM3 results in rRNA maturation defects, altered nucleolar morphology, and reduced ribosome production. Beyond ribosome biogenesis, PUM3 may also modulate mRNA stability and translation through interactions with additional RNA-binding proteins and components of the exosome complex.\u003cbr\u003e\u003cbr\u003eThe PUM3 antibody is used in molecular biology, RNA regulation, and ribosome assembly research to detect Pumilio homolog 3 protein and analyze its cellular localization. Western blot analysis identifies a 74 kilodalton band, while immunofluorescence reveals prominent nucleolar staining overlapping with fibrillarin. Because of its role in nucleolar RNA metabolism, this antibody provides a tool to study gene expression regulation at the post-transcriptional level.\u003cbr\u003e\u003cbr\u003eRecent studies suggest that PUM3 interacts with factors controlling RNA stability and surveillance, positioning it as a regulator of RNA quality control. The PUM3 antibody facilitates the exploration of these processes and supports studies of nucleolar function, gene expression, and RNA-protein network organization.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PUM3 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PUM3 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PUM3 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PUM3 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PUM3+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047299375469,"sku":"FY12760","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_66c1dacf-0cbd-4504-9fa9-67e2fa5df56c.jpg?v=1782237044","url":"https:\/\/www.ebiohippo.com\/products\/pum3-antibody-pumilio-3-bha17135663","provider":"BioHippo","version":"1.0","type":"link"}