{"product_id":"puromycin-bhb21300290","title":"Puromycin","description":"\u003ch3\u003eProduct details\u003c\/h3\u003e \u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eChemical name:\u003c\/strong\u003e 3'-[alpha-Amino-p-methoxyhydrocinnamamido]-3'-deoxy-N,N-dimethyladenosine dihydrochloride.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAlso known as:\u003c\/strong\u003e Puromycin dihydrochloride, Stylomycin, P638\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCAS number:\u003c\/strong\u003e 58-58-2\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMolecular formula:\u003c\/strong\u003e C22H31Cl2N7O5 (C22H29N7O5·2HCl).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurity:\u003c\/strong\u003e \u0026gt;99% (HPLC)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eConcentration:\u003c\/strong\u003e 2-60 µM.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eForm:\u003c\/strong\u003e Lyophilized powder.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSolubility:\u003c\/strong\u003e Water. Centrifuge all product preparations before use (10000 x g 5 min).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReconstitution:\u003c\/strong\u003e Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is lyophilized in 0.5 ml conical vial. The product is soluble in pure water to high-micromolar concentrations (5 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity. Avoid exposure to light.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eShipping:\u003c\/strong\u003e Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSource:\u003c\/strong\u003e Natural\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eActivity:\u003c\/strong\u003e Puromycin is an antibiotic known to inhibit protein synthesis1, as well as an apoptosis inducer2. Puromycin can be used as a selection agent for mammalian cells, transformed to express puromycin-N-acetyl-transferase, at concentrations ranging between 0.5-2 µg\/ml for suspension cell lines, and 2-6 µg\/ml for adherent cell lines.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAlternative names:\u003c\/strong\u003e Puromycin dihydrochloride, Stylomycin, P638\u003c\/li\u003e\n\u003c\/ul\u003e \u003ch3\u003eScientific background\u003c\/h3\u003e \u003cp\u003ePuromycin is an aminoacyl nucleoside antibiotic produced by Streptomyces alboniger. It is a broad spectrum, non-specific inhibitor of protein synthesis in Gram-positive bacteria, protozoans, and mammalian cells, including tumor cells, and is widely used as a key compound in various cell-free systems directed to elucidate the mechanism of protein synthesis and the mode of action of other inhibitors of this process.1Puromycin is an analog for the 3' terminal of Tyr-tRNATyr which inhibits the enzyme peptidyl-transferase located in the 30s ribosomal subunits. Puromycin inhibits the enzyme by competition with aminoacyl-tRNA on the binding to the A' site or by attacking the peptidyl-tRNA in the P site of the enzyme. By this, the growing peptide chains on ribosomes are linked to the α-amino group of puromycin and interrupt the normal reaction of peptide bond formation.2-6Low levels of puromycin were found to act as an inhibitor of the soluble aminopeptidase (puromycin-sensitive aminopeptidase-PSA) in a variety of tissues. PSA inhibition by low puromycin concentrations that do not interfere with protein synthesis may directly lead to cell cycle arrest at the G2\/M phase and induction of apoptosis.7,8\u003c\/p\u003e \u003cp\u003e\u003cstrong\u003eLead time:\u003c\/strong\u003e 1-2 Business Days\u003c\/p\u003e \u003cp\u003e\u003cstrong\u003eCountry of origin:\u003c\/strong\u003e Israel\/IL\u003c\/p\u003e \u003ch3\u003eApplications key\u003c\/h3\u003e \u003cp\u003eApplication key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.\u003c\/p\u003e \u003cp\u003e\u003cstrong\u003eBioassay tested:\u003c\/strong\u003e Yes\u003c\/p\u003e","brand":"Alomone Labs","offers":[{"title":"1 g \/ 1","offer_id":53249713144173,"sku":"P-540-1G-1","price":849.0,"currency_code":"USD","in_stock":true},{"title":"100 mg \/ 1","offer_id":53311573000557,"sku":"P-540-100MG-1","price":117.0,"currency_code":"USD","in_stock":true},{"title":"250 mg \/ 1","offer_id":53311573033325,"sku":"P-540-250MG-1","price":263.0,"currency_code":"USD","in_stock":true},{"title":"50 mg \/ 1","offer_id":53311573066093,"sku":"P-540-50MG-1","price":66.0,"currency_code":"USD","in_stock":true},{"title":"500 mg \/ 1","offer_id":53311573098861,"sku":"P-540-500MG-1","price":476.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/P-540.gif?v=1778544446","url":"https:\/\/www.ebiohippo.com\/products\/puromycin-bhb21300290","provider":"BioHippo","version":"1.0","type":"link"}