{"product_id":"pxre-reporter-lentivirus-bhv19400223","title":"PXRE Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003ePXR (Pregnane X Receptor, NR1I2, also called SXR) is a ligand-activated nuclear receptor that serves as a master regulator of xenobiotic and drug metabolism. Activated by a broad range of structurally diverse compounds, PXR heterodimerizes with the retinoid X receptor and binds PXR response elements (PXRE) in the promoters of genes encoding drug-metabolizing enzymes and transporters, most notably the cytochrome P450 enzyme CYP3A4. PXR is highly expressed in liver and intestine, where it coordinates the body's defense against potentially harmful chemicals. Because PXR governs drug clearance and underlies many drug-drug interactions, it is a key target in pharmacology and toxicology research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe PXRE Reporter Lentivirus is a transcription-factor reporter system for detecting PXR-mediated transcriptional activity in mammalian cells. The construct uses tandem repeats of the PXR response element from the CYP3A4 promoter, arranged in an ER6 configuration, coupled to a minimal promoter that drives a fluorescent or luminescent reporter, so the readout reflects activation by PXR\/RXR heterodimers. A constitutively expressed selection marker and optional secondary reporter support stable polyclonal reporter cell lines.\u003c\/p\u003e\n\u003cp\u003eStable lentiviral integration provides consistent reporter expression in dividing and post-mitotic cells, including primary and cryopreserved cultures, avoiding transient-transfection variability. Particles are purified by PEG precipitation and sucrose gradient centrifugation and transduce difficult-to-transfect cells, supporting research on xenobiotic metabolism, CYP3A4 induction, and drug-drug interaction screening.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a d2GFP, EGFP, Firefly Luc, GFP + Firefly Luc, mCherry, Renilla Luc, RFP + Firefly Luc, BFP2, Gaussia Luc, GFP, RFP reporter gene under the control of tandem consensus response elements specific for the Pregnane X receptor (PXR) transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Hygromycin, Puromycin, Zeocin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53251625156973,"sku":"LTV-0114-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53362027299181,"sku":"LTV-0114-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Puromycin \/ 2x10^6","offer_id":53362027331949,"sku":"LTV-0114-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Blasticidin \/ 2x10^6","offer_id":53362027364717,"sku":"LTV-0114-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53362027397485,"sku":"LTV-0114-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53362027430253,"sku":"LTV-0114-6S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/PXRE-TAG-PURO-fig1.jpg?v=1776934141","url":"https:\/\/www.ebiohippo.com\/products\/pxre-reporter-lentivirus-bhv19400223","provider":"BioHippo","version":"1.0","type":"link"}