{"product_id":"pycr3-antibody-pyrroline-5-carboxylate-reductase-3-bha17135562","title":"PYCR3 Antibody \/ Pyrroline-5-carboxylate reductase 3","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePYCR3 Antibody \/ Pyrroline-5-carboxylate reductase 3 is a anti-PYCR3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PYCR3\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePYCR3 antibody detects Pyrroline-5-carboxylate reductase 3, a mitochondrial enzyme that catalyzes the NADH-dependent reduction of pyrroline-5-carboxylate to proline, completing the final step of proline biosynthesis. PYCR3 plays a key role in amino acid metabolism, redox regulation, and cell growth. The PYCR3 antibody is widely used in cancer metabolism, mitochondrial biology, and redox signaling research to study proline synthesis and metabolic adaptation.\u003cbr\u003e\u003cbr\u003ePYCR3 is encoded by the PYCR3 gene located on human chromosome 8q24.3. The protein is approximately 319 amino acids long and localizes to the mitochondrial matrix, functioning alongside PYCR1 and PYCR2 in the proline biosynthetic pathway. PYCR3 preferentially uses NADH as a cofactor and contributes to redox homeostasis by balancing NAD+\/NADH ratios within mitochondria.\u003cbr\u003e\u003cbr\u003eThe PYCR3 antibody detects a 33 kilodalton band by western blot and shows punctate mitochondrial localization under fluorescence microscopy. Through its regulation of proline metabolism, PYCR3 supports anabolic growth, oxidative stress resistance, and survival under nutrient deprivation. It also influences collagen synthesis and extracellular matrix remodeling by controlling intracellular proline availability.\u003cbr\u003e\u003cbr\u003ePYCR3 expression is upregulated in several cancers, including colorectal, lung, and prostate carcinoma, where it promotes tumor cell proliferation and metastasis by maintaining mitochondrial NADH pools. Knockdown of PYCR3 reduces ATP production, increases reactive oxygen species, and triggers apoptosis, demonstrating its role in metabolic flexibility. Outside of cancer, PYCR3 supports fibroblast function and tissue repair by providing proline for collagen biosynthesis.\u003cbr\u003e\u003cbr\u003eAs a regulator of both amino acid metabolism and mitochondrial redox balance, PYCR3 represents a metabolic control point linking biosynthesis and energy maintenance.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PYCR3 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PYCR3 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PYCR3 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PYCR3 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PYCR3+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047295836525,"sku":"FY12659","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_5c36cae5-580b-47b1-9de0-b09eccefb583.jpg?v=1782237028","url":"https:\/\/www.ebiohippo.com\/products\/pycr3-antibody-pyrroline-5-carboxylate-reductase-3-bha17135562","provider":"BioHippo","version":"1.0","type":"link"}