{"product_id":"pyhin1-antibody-pyrin-and-hin-domain-containing-protein-1-bha17135658","title":"PYHIN1 Antibody \/ Pyrin and HIN domain-containing protein 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePYHIN1 Antibody \/ Pyrin and HIN domain-containing protein 1 is a anti-PYHIN1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PYHIN1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC, IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePYHIN1 antibody detects Pyrin and HIN domain-containing protein 1, a nuclear and cytoplasmic signaling protein involved in immune regulation, cell cycle control, and apoptosis. Encoded by the PYHIN1 gene on chromosome 1q23.1, this protein belongs to the HIN-200 (hematopoietic interferon-inducible nuclear) family of interferon-inducible proteins, which are characterized by an N-terminal pyrin domain and a C-terminal HIN-200 DNA-binding domain. These domains mediate protein-protein and protein-DNA interactions that regulate immune transcription and DNA damage response pathways.\u003cbr\u003e\u003cbr\u003ePYHIN1 functions primarily as a transcriptional regulator and sensor of foreign or damaged DNA. It can bind double-stranded DNA through its HIN domain and modulate transcription of interferon-stimulated genes. In the nucleus, PYHIN1 interacts with transcriptional co-regulators to influence gene expression linked to antiviral defense and tumor suppression. In the cytoplasm, it contributes to inflammasome regulation and apoptotic signaling. Expression of PYHIN1 is strongly induced by type I interferons and inflammatory cytokines, linking it to host defense and immune surveillance.\u003cbr\u003e\u003cbr\u003eThe PYHIN1 antibody is used in immunology, oncology, and molecular biology to investigate DNA sensing, innate immune activation, and cell cycle regulation. Western blot analysis identifies a 74 kilodalton band corresponding to PYHIN1, while immunofluorescence shows both nuclear and cytoplasmic staining depending on cell type and activation state. Because PYHIN1 acts at the interface of DNA sensing and transcriptional control, the antibody is a valuable tool for studying interferon signaling and genomic stability mechanisms.\u003cbr\u003e\u003cbr\u003eBeyond innate immunity, PYHIN1 plays roles in tumor suppression and apoptosis. Its expression inhibits cell proliferation by interfering with E2F transcriptional activity and promoting p53-dependent growth arrest. Loss of PYHIN1 has been reported in several cancers, suggesting that it functions as a tumor suppressor gene. The PYHIN1 antibody enables researchers to explore these functions and track its expression across different physiological and disease contexts.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PYHIN1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PYHIN1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PYHIN1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PYHIN1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PYHIN1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047299244397,"sku":"FY12755","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_c52ee385-17a9-4fc4-a11a-4694fae5e31a.jpg?v=1772019337","url":"https:\/\/www.ebiohippo.com\/products\/pyhin1-antibody-pyrin-and-hin-domain-containing-protein-1-bha17135658","provider":"BioHippo","version":"1.0","type":"link"}