{"product_id":"qki-antibody-protein-quaking-bha17135608","title":"QKI Antibody \/ Protein Quaking","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eQKI Antibody \/ Protein Quaking is a anti-QKI Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat, Monkey. Reported localization: Nuclear, cytoplasmic.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e QKI\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC\/IF, IP, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eQKI antibody detects Protein quaking (also known as KH domain-containing RNA-binding protein QKI), a member of the STAR (Signal Transduction and Activation of RNA) family of RNA-binding proteins that regulate mRNA splicing, export, stability, and translation. Encoded by the QKI gene on chromosome 6q26, this protein plays an essential role in myelination, neural development, and cell differentiation. QKI contains a single KH (hnRNP K homology) RNA-binding domain and functions as both a translational repressor and splicing modulator depending on cellular context. The protein binds specific RNA motifs known as Quaking Response Elements (QREs), influencing alternative splicing patterns in numerous genes that control cytoskeletal dynamics and myelin sheath formation.\u003cbr\u003e\u003cbr\u003eQKI exists in multiple isoforms�QKI-5, QKI-6, and QKI-7�generated through alternative splicing of the C-terminal region. These isoforms differ in subcellular localization and function: QKI-5 resides predominantly in the nucleus and regulates pre-mRNA splicing, QKI-6 shuttles between the nucleus and cytoplasm to coordinate mRNA export and translation, and QKI-7 localizes mainly in the cytoplasm where it modulates mRNA stability. Collectively, these isoforms maintain neuronal and glial cell homeostasis. Mutations or loss of QKI disrupt oligodendrocyte maturation and cause hypomyelination, while abnormal expression contributes to neurodevelopmental disorders and gliomas.\u003cbr\u003e\u003cbr\u003eThe QKI antibody is widely used in neuroscience and RNA biology research to study RNA metabolism and myelin formation. Western blot analysis detects bands corresponding to the major QKI isoforms ranging from 38 to 45 kilodaltons. Immunofluorescence with this antibody reveals nuclear and cytoplasmic localization patterns depending on isoform distribution and cell type. In the central nervous system, QKI is expressed in oligodendrocytes, astrocytes, and neurons, where it modulates the expression of myelin-related genes such as MBP and PLP1. This makes the QKI antibody a valuable reagent for investigating myelination, glial cell biology, and post-transcriptional regulation.\u003cbr\u003e\u003cbr\u003eBeyond the nervous system, QKI influences epithelial-mesenchymal transition, vascular smooth muscle differentiation, and cardiac development. It has also been implicated in tumor suppression, with reduced expression linked to glioblastoma and colorectal cancer progression. The protein interacts with signaling molecules including STAT1 and AKT, suggesting integration between RNA processing and signal transduction. Researchers employ the QKI antibody to monitor these molecular pathways, exploring how altered RNA regulation drives disease phenotypes.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=QKI - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=QKI - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=QKI - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/QKI - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=QKI+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047296688493,"sku":"FY12705","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_fd8063e7-d9cf-42bc-aafc-989e5f29b8b3.jpg?v=1782237032","url":"https:\/\/www.ebiohippo.com\/products\/qki-antibody-protein-quaking-bha17135608","provider":"BioHippo","version":"1.0","type":"link"}