{"product_id":"quantichrom-arginase-inhibitor-screening-kit-bht15600223","title":"QuantiChrom™ Arginase Inhibitor Screening Kit","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eFor evaluation and high-throughput screen (HTS) of arginase modulators. The assay uses OD430nm for signal readout. Compatible sample input includes Compounds that affect arginase activity. Typical stated assay timing is 1 hour 45 min.\u003c\/p\u003e\n\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003e\n\u003cstrong\u003eReadout format:\u003c\/strong\u003e OD430nm supports plate-based signal acquisition and consistent comparison across matched samples.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eSample compatibility:\u003c\/strong\u003e The stated sample scope includes Compounds that affect arginase activity, which is useful when aligning matrix type with calibration and control design.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eWorkflow timing:\u003c\/strong\u003e The listed assay time of 1 hour 45 min helps frame batch planning, replicate handling, and plate throughput.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eFeature emphasis:\u003c\/strong\u003e Safe. Non-radioactive assay.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eAdditional feature notes highlight High-throughput. Homogenous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling system; Rapid and reliable. Can be completed in less than 2 hours and no 37°C heater is needed. Available format information for this listing includes 100 Tests (Custom bulk sizes available upon request).\u003c\/p\u003e\n\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eThis product is centered on measurement of arginase inhibitor screening within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.\u003c\/p\u003e\n\n\u003ch2\u003eMore details\u003c\/h2\u003e\n\u003cp\u003eARGINASE (L-arginine ureohydrolase EC 3.5.3.1) is present in mammals and plants. In humans, arginase is expressed predominantly in the liver, and to lesser degrees in the breast, kidney, testes, salivary glands, epidermis and erythrocytes. Arginase catalyzes the conversion of arginine to ornithine and urea, important for protection against NH 3 toxicity and for cell growth and repair. Excessive arginase activity has been linked to cardiovascular diseases and also contributes to vascular structural problems and neural toxicity. Studies show that arginase inhibitors have been proven to be beneficial in cardiovascular and nervous system diseases. Simple, direct, and automation-ready procedures for measuring arginase inhibition are highly desirable in Research and Drug Discovery.BioAssay Systems’ arginase inhibitor screening kit provides a sensitive and convenient method to screen for arginase inhibitors. The method utilizes a chromogen that forms a colored complex specifically with urea produced in the arginase reaction. The intensity of the color is directly proportional to the arginase activity in the sample. Percent inhibition of a test compound can be determined by comparing the color intensity of the reaction preincubated with the test compound with the color intensity of an untreated control reaction\u003c\/p\u003e\n\n\u003ch2\u003eDetection method\u003c\/h2\u003e\n\u003cp\u003eColorimetric (OD 430 nm).\u003c\/p\u003e\n\n\u003ch2\u003eProcedures and timing\u003c\/h2\u003e\n\u003cp\u003eStated procedure or timing information: 1 hour 45 min.\u003c\/p\u003e\n\n\u003ch2\u003eScreening services\u003c\/h2\u003e\n\u003cp\u003eBioAssay Systems offers comprehensive Arginase lead discovery services, including compound library screening, inhibitor profiling, and high-throughput assays to identify potential drug candidates. We provide detailed analyses of compound activity, potency, and selectivity, supporting hit-to-lead and lead optimization efforts. Please contact us by Service .\u003c\/p\u003e\n\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003ePlate-based quantification and side-by-side group comparison remain central use cases for this assay format.\u003c\/li\u003e\n  \u003cli\u003eThe product notes emphasize multi-sample throughput, making it relevant for screening-oriented and larger batch comparison studies.\u003c\/li\u003e\n  \u003cli\u003eThe description supports intervention-focused study designs in which researchers compare baseline and perturbed conditions.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eQuantify arginase inhibitor screening in compounds that affect arginase activity by OD430 nm readout.\u003c\/li\u003e\n  \u003cli\u003eCompare treatment or phenotype groups using matched compounds that affect arginase activity handling.\u003c\/li\u003e\n  \u003cli\u003eMonitor time-course or pre\/post changes in compounds that affect arginase activity across study conditions.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eInterpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.\u003c\/p\u003e\n\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eMatrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.\u003c\/li\u003e\n  \u003cli\u003eUse appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c!-- Sources (internal):\n- Product Description column\n- Key Features column\n- More Details column\n- Method \/ Sample Type(s) \/ Assay Time \/ Detection Limit \/ Detection Method columns\n- Procedures column\n- Screening Services column\n--\u003e","brand":"BioAssay Systems","offers":[{"title":"100 Tests (Custom bulk sizes available upon request)","offer_id":53238320890221,"sku":"IARG-100","price":318.27,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/IARGfig.jpg?v=1776668362","url":"https:\/\/www.ebiohippo.com\/products\/quantichrom-arginase-inhibitor-screening-kit-bht15600223","provider":"BioHippo","version":"1.0","type":"link"}