{"product_id":"quantichrom-beta-glucuronidase-assay-kit-bht15600043","title":"QuantiChrom™ β-Glucuronidase Assay Kit","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eFor quantitative determination of β-glucuronidase enzyme activity in biological samples, e.g. blood, tissue and bacterial samples. The assay uses FL365\/450nm for signal readout. Compatible sample input includes Blood, tissue and bacterial samples. Typical stated assay timing is 30 min.\u003c\/p\u003e\n\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003e\n\u003cstrong\u003eReadout format:\u003c\/strong\u003e FL365\/450nm supports plate-based signal acquisition and consistent comparison across matched samples.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eSample compatibility:\u003c\/strong\u003e The stated sample scope includes Blood, tissue and bacterial samples, which is useful when aligning matrix type with calibration and control design.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eAnalytical range context:\u003c\/strong\u003e The supplied specifications include a stated detection limit of 10 -4 U\/L for interpreting low-signal samples.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eFeature emphasis:\u003c\/strong\u003e Highly Sensitive. The fluorimetric nature of the kit detects 1×10 -4 U\/L to 8 U\/L BG activity in 96 well plate with only 10 µL sample within 30 min.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eAdditional feature notes highlight Versatile. This assay has been validated to work with both animal and E. coli derived β-glucuronidase; High-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated to assay thousands of samples per day. Available format information for this listing includes 100 Tests in 96-well plate.\u003c\/p\u003e\n\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eThis product is centered on measurement of β-glucuronidase within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.\u003c\/p\u003e\n\n\u003ch2\u003eMore details\u003c\/h2\u003e\n\u003cp\u003e\u003ci\u003eBETA-GLUCURONIDASE (BG) \u003c\/i\u003eis an enzyme which catalyzes the hydrolysis of β-glucuronide bonds. In humans, β-glucuronidase catalyzes the hydrolysis of β-D-glucuronic acids from glycoproteins and mucopolysaccharides such as heparan sulfate. β-glucuronidase activity is important for drug metabolism as drugs are often conjugated to glucuronic acid to make for a more water soluble delivery system, relying on subsequent β-glucuronidase activity to release the active drug. Bilirubin clearance is also facilitated by glucuronidation. GUSB is often used as a reporter gene to track gene expression through β-glucuronidase activity. BioAssay Systems’ fluorimetric BG assay uses a β-D-glucuronide substrate which fluoresces upon hydrolysis with β-glucuronidase and is read at 365\/450 nm.\u003c\/p\u003e\n\n\u003ch2\u003eDetection method\u003c\/h2\u003e\n\u003cp\u003eFluorescent (FL 365\/450 nm).\u003c\/p\u003e\n\n\u003ch2\u003eDetection limit and analytical sensitivity\u003c\/h2\u003e\n\u003cp\u003eReported detection limit: 10 -4 U\/L.\u003c\/p\u003e\n\n\u003ch2\u003eProcedures and timing\u003c\/h2\u003e\n\u003cp\u003eStated procedure or timing information: 30 min.\u003c\/p\u003e\n\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003ePlate-based quantification and side-by-side group comparison remain central use cases for this assay format.\u003c\/li\u003e\n  \u003cli\u003eThe product notes emphasize multi-sample throughput, making it relevant for screening-oriented and larger batch comparison studies.\u003c\/li\u003e\n  \u003cli\u003eShort assay timing and plate compatibility support time-course or repeated-measure collection plans when handling is kept consistent.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eQuantify β-glucuronidase in blood, tissue and bacterial samples by FL365\/450 nm readout.\u003c\/li\u003e\n  \u003cli\u003eCompare treatment or phenotype groups using matched blood, tissue and bacterial samples handling.\u003c\/li\u003e\n  \u003cli\u003eMonitor time-course or pre\/post changes in blood, tissue and bacterial samples across study conditions.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eInterpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.\u003c\/p\u003e\n\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eMatrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.\u003c\/li\u003e\n  \u003cli\u003eUse appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c!-- Sources (internal):\n- Product Description column\n- Key Features column\n- More Details column\n- Method \/ Sample Type(s) \/ Assay Time \/ Detection Limit \/ Detection Method columns\n- Procedures column\n- Screening Services column\n--\u003e","brand":"BioAssay Systems","offers":[{"title":"100 Tests in 96-well plate","offer_id":53238316138861,"sku":"DGCD-100","price":469.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/DGCDfig.jpg?v=1776668352","url":"https:\/\/www.ebiohippo.com\/products\/quantichrom-beta-glucuronidase-assay-kit-bht15600043","provider":"BioHippo","version":"1.0","type":"link"}