{"product_id":"quantichrom-citrate-synthase-assay-kit-bht15600033","title":"QuantiChrom™ Citrate Synthase Assay Kit","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eDirect assays of citrate synthase activity in cell lysates, tissues, and other biological samples. Linear detection range 0.50 to 120 U\/L citrate synthase activity in 96-well plate assay. Simple and convenient. The procedure involves. The assay uses OD412nm for signal readout. Compatible sample input includes Cell lysates, tissues, and other biological samples. Typical stated assay timing is 10 min.\u003c\/p\u003e\n\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003e\n\u003cstrong\u003eReadout format:\u003c\/strong\u003e OD412nm supports plate-based signal acquisition and consistent comparison across matched samples.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eSample compatibility:\u003c\/strong\u003e The stated sample scope includes Cell lysates, tissues, and other biological samples, which is useful when aligning matrix type with calibration and control design.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eAnalytical range context:\u003c\/strong\u003e The supplied specifications include a stated detection limit of 0.50 U\/L for interpreting low-signal samples.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eFeature emphasis:\u003c\/strong\u003e Sensitive and accurate. Linear detection range 0.50 to 120 U\/L citrate synthase activity in 96-well plate assay.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eAdditional feature notes highlight Simple and convenient. The procedure involves adding a single working reagent and reading the optical density at 5 min and 10 min at room temperature; High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Available format information for this listing includes 100 Tests in 96-well plate.\u003c\/p\u003e\n\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eThis product is centered on measurement of citrate synthase within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.\u003c\/p\u003e\n\n\u003ch2\u003eMore details\u003c\/h2\u003e\n\u003cp\u003e\u003ci\u003eCITRATE SYNTHASE\u003c\/i\u003e(E.C. 2.3.3.1) is a pivotal enzyme that initiates the tricarboxylic acid (TCA) cycle. It catalyzes the condensation reaction between acetyl-CoA and oxaloacetic acid, yielding citric acid. Citrate synthase levels of cells and tissue are used as a guide in determining mitochondrial dysfunctions and cellular metabolism. High levels of citrate synthase activity indicate increased metabolic activity or cellular energy production. This can occur in situations where cells are actively metabolizing substrates for energy production, such as during periods of increased physical activity or in tissues with high energy demands, like muscle tissue. Conversely, low levels of citrate synthase activity may suggest reduced metabolic activity or energy production. This could be seen in situations where cellular metabolism is slowed down, such as during periods of rest or in conditions associated with mitochondrial dysfunction. Simple, direct and automation-ready procedures for measuring citrate synthase activity are very desirable. BioAssay Systems’ QuantiChrom™ Citrate Synthase Assay is based on an improved Ellman method, in which coenzyme A thiol produced by the action of citrate synthase forms a yellow color with 5,5′-dithiobis (2-nitrobenzoic acid). The intensity of the product color, measured at 412 nm, is proportionate to the enzyme activity in the sample.\u003c\/p\u003e\n\n\u003ch2\u003eDetection method\u003c\/h2\u003e\n\u003cp\u003eColorimetric (OD 412 nm).\u003c\/p\u003e\n\n\u003ch2\u003eDetection limit and analytical sensitivity\u003c\/h2\u003e\n\u003cp\u003eReported detection limit: 0.50 U\/L.\u003c\/p\u003e\n\n\u003ch2\u003eProcedures and timing\u003c\/h2\u003e\n\u003cp\u003eStated procedure or timing information: 10 min.\u003c\/p\u003e\n\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003ePlate-based quantification and side-by-side group comparison remain central use cases for this assay format.\u003c\/li\u003e\n  \u003cli\u003eThe product notes emphasize multi-sample throughput, making it relevant for screening-oriented and larger batch comparison studies.\u003c\/li\u003e\n  \u003cli\u003eShort assay timing and plate compatibility support time-course or repeated-measure collection plans when handling is kept consistent.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eQuantify citrate synthase in cell lysates, tissues by OD412 nm readout.\u003c\/li\u003e\n  \u003cli\u003eCompare treatment or phenotype groups using matched cell lysates, tissues handling.\u003c\/li\u003e\n  \u003cli\u003eMonitor time-course or pre\/post changes in cell lysates, tissues across study conditions.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eInterpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.\u003c\/p\u003e\n\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eMatrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.\u003c\/li\u003e\n  \u003cli\u003eUse appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c!-- Sources (internal):\n- Product Description column\n- Key Features column\n- More Details column\n- Method \/ Sample Type(s) \/ Assay Time \/ Detection Limit \/ Detection Method columns\n- Procedures column\n- Screening Services column\n--\u003e","brand":"BioAssay Systems","offers":[{"title":"100 Tests in 96-well plate","offer_id":53238312403309,"sku":"DCST-100","price":489.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/DCSTfig.jpg?v=1776668349","url":"https:\/\/www.ebiohippo.com\/products\/quantichrom-citrate-synthase-assay-kit-bht15600033","provider":"BioHippo","version":"1.0","type":"link"}