{"product_id":"quantichrom-dpph-antioxidant-capacity-assay-kit-bht15600022","title":"QuantiChrom™ DPPH Antioxidant Capacity Assay Kit","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eFor quantitative determination of antioxidant capacity in food, beverages and biological samples. Sensitive and accurate. Detects 11 µM to 300 µM Trolox equivalents in 96-well plate. Stable reagent. DPPH in this kit is included as a powder. The assay uses OD517nm for signal readout. Compatible sample input includes Food, beverages, and biological samples. Typical stated assay timing is 10 min.\u003c\/p\u003e\n\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003e\n\u003cstrong\u003eReadout format:\u003c\/strong\u003e OD517nm supports plate-based signal acquisition and consistent comparison across matched samples.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eSample compatibility:\u003c\/strong\u003e The stated sample scope includes Food, beverages, and biological samples, which is useful when aligning matrix type with calibration and control design.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eAnalytical range context:\u003c\/strong\u003e The supplied specifications include a stated detection limit of 11 μM for interpreting low-signal samples.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eFeature emphasis:\u003c\/strong\u003e Sensitive and accurate. Detects 11 μM to 300 μM Trolox equivalents in 96-well plate.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eAdditional feature notes highlight Stable reagent. DPPH in this kit is included as a powder form that can be aliquoted to test as many or few wells as desired, or repeated testing over separate days, or weeks; Simple and high-throughput. This room temperature, homogenous, “mix-incubate-measure” assay can be run in as little as 10 minutes. Can be readily automated to assay thousands of samples per day. Available format information for this listing includes 120 Tests in 96-well plate.\u003c\/p\u003e\n\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eThis product is centered on measurement of dpph antioxidant capacity within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.\u003c\/p\u003e\n\n\u003ch2\u003eMore details\u003c\/h2\u003e\n\u003cp\u003eAn\u003ci\u003eANTIOXIDANT\u003c\/i\u003eis a molecule capable of slowing or preventing the oxidation of other molecules. Antioxidants, such as the small molecules glutathione and vitamins or the macromolecules catalase and glutathione peroxidase, protect the cells from damage by reactive oxygen species. Antioxidants are widely used as dietary supplements and in industry as preservatives in food, cosmetics, rubber and gasoline.Simple, direct and high-throughput assays for antioxidant capacity find wide applications in research, food industry and drug discovery. BioAssay Systems’ improved antioxidant capacity assay is based on the reduction of DPPH by antioxidant molecules in a sample, which results in a drop in absorbance at 517 nm. The antioxidant capacity can be quantified and expressed using a Trolox standard.\u003c\/p\u003e\n\n\u003ch2\u003eDetection method\u003c\/h2\u003e\n\u003cp\u003eColorimetric (OD 517 nm).\u003c\/p\u003e\n\n\u003ch2\u003eDetection limit and analytical sensitivity\u003c\/h2\u003e\n\u003cp\u003eReported detection limit: 11 μM.\u003c\/p\u003e\n\n\u003ch2\u003eProcedures and timing\u003c\/h2\u003e\n\u003cp\u003eStated procedure or timing information: 10 min.\u003c\/p\u003e\n\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003ePlate-based quantification and side-by-side group comparison remain central use cases for this assay format.\u003c\/li\u003e\n  \u003cli\u003eThe product notes emphasize multi-sample throughput, making it relevant for screening-oriented and larger batch comparison studies.\u003c\/li\u003e\n  \u003cli\u003eShort assay timing and plate compatibility support time-course or repeated-measure collection plans when handling is kept consistent.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eQuantify dpph antioxidant capacity in food, beverages, and biological samples by OD517 nm readout.\u003c\/li\u003e\n  \u003cli\u003eCompare treatment or phenotype groups using matched food, beverages, and biological samples handling.\u003c\/li\u003e\n  \u003cli\u003eMonitor time-course or pre\/post changes in food, beverages, and biological samples across study conditions.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eInterpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.\u003c\/p\u003e\n\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eMatrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.\u003c\/li\u003e\n  \u003cli\u003eUse appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c!-- Sources (internal):\n- Product Description column\n- Key Features column\n- More Details column\n- Method \/ Sample Type(s) \/ Assay Time \/ Detection Limit \/ Detection Method columns\n- Procedures column\n- Screening Services column\n--\u003e","brand":"BioAssay Systems","offers":[{"title":"120 Tests in 96-well plate","offer_id":53238315876717,"sku":"DAOC-120","price":513.97,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/DAOCfig.jpg?v=1776668353","url":"https:\/\/www.ebiohippo.com\/products\/quantichrom-dpph-antioxidant-capacity-assay-kit-bht15600022","provider":"BioHippo","version":"1.0","type":"link"}