{"product_id":"quantichrom-fatty-acid-uptake-assay-kit-bht15600038","title":"QuantiChrom™ Fatty Acid Uptake Assay Kit","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eFor quantitative determination of long-chain fatty acid uptake in whole cells and evaluation of effects of ligands or drugs on fatty acid transport. The assay uses FL 488\/523nm for signal readout. Compatible sample input includes Adipocytes and other fatty acid-transporting cells. Or compounds that affect fatty acid uptake activity. Typical stated assay timing is 2 hrs.\u003c\/p\u003e\n\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003e\n\u003cstrong\u003eReadout format:\u003c\/strong\u003e FL 488\/523nm supports plate-based signal acquisition and consistent comparison across matched samples.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eSample compatibility:\u003c\/strong\u003e The stated sample scope includes Adipocytes and other fatty acid-transporting cells. Or compounds that affect fatty acid uptake activity, which is useful when aligning matrix type with calibration and control design.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eWorkflow timing:\u003c\/strong\u003e The listed assay time of 2 hrs helps frame batch planning, replicate handling, and plate throughput.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eFeature emphasis:\u003c\/strong\u003e Safe. Non-radioactive assay.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eAdditional feature notes highlight Fast and Sensitive. Homogenous “add-and-read” assay. No wash, lysis, or staining steps are needed; Simple and Convenient. Can be automated as a high-throughput assay for fatty acid transport and modulator screens in cells. Available format information for this listing includes 100 Tests.\u003c\/p\u003e\n\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eThis product is centered on measurement of fatty acid uptake within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.\u003c\/p\u003e\n\n\u003ch2\u003eMore details\u003c\/h2\u003e\n\u003cp\u003e\u003ci\u003eLONG CHAIN FATTY ACIDS \u003c\/i\u003e(LCFA) are important fuel sources for animals as substrates in β-oxidation and serve as building blocks for many different cellular structures. Long-chain unesterified fatty acids (LCFA) are transported into cells using membrane transport proteins, and increased LCFA levels in cells are common in diabetes, obesity-related diseases, cardiovascular disease, and certain forms of cancer. Therefore, fatty acid uptake is a significant therapeutic target for the treatment of metabolic disorders and an important topic for metabolic research.BioAssay Systems’ fluorescent cell-based fatty acid uptake assay uses a fluorescent fatty acid analog which is taken up by fatty acid transporter proteins and accumulates within the cell. Quench reagent is added to block extracellular fluorescent signals in the medium. The adherent cells import the fatty acid analog, and the bottom-read fluorimeter measures the increase in fluorescence signal at λex\/em = 488\/523nm. This high-throughput assay can be applied to assess fatty acid uptake activity in cells and to screen for activators and inhibitors.\u003c\/p\u003e\n\n\u003ch2\u003eDetection method\u003c\/h2\u003e\n\u003cp\u003eFluorescent (FL 488\/523 nm).\u003c\/p\u003e\n\n\u003ch2\u003eProcedures and timing\u003c\/h2\u003e\n\u003cp\u003eStated procedure or timing information: 2 hrs.\u003c\/p\u003e\n\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003ePlate-based quantification and side-by-side group comparison remain central use cases for this assay format.\u003c\/li\u003e\n  \u003cli\u003eThe product notes emphasize multi-sample throughput, making it relevant for screening-oriented and larger batch comparison studies.\u003c\/li\u003e\n  \u003cli\u003eThe description supports intervention-focused study designs in which researchers compare baseline and perturbed conditions.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eQuantify fatty acid uptake in adipocytes and other fatty acid-transporting by FL 488\/523 nm readout.\u003c\/li\u003e\n  \u003cli\u003eCompare treatment or phenotype groups using matched adipocytes and other fatty acid-transporting handling.\u003c\/li\u003e\n  \u003cli\u003eMonitor time-course or pre\/post changes in adipocytes and other fatty acid-transporting across study conditions.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eInterpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.\u003c\/p\u003e\n\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eMatrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.\u003c\/li\u003e\n  \u003cli\u003eUse appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c!-- Sources (internal):\n- Product Description column\n- Key Features column\n- More Details column\n- Method \/ Sample Type(s) \/ Assay Time \/ Detection Limit \/ Detection Method columns\n- Procedures column\n- Screening Services column\n--\u003e","brand":"BioAssay Systems","offers":[{"title":"100 Tests","offer_id":53238313353581,"sku":"DFFU-100","price":499.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/DFFUfig.jpg?v=1776668352","url":"https:\/\/www.ebiohippo.com\/products\/quantichrom-fatty-acid-uptake-assay-kit-bht15600038","provider":"BioHippo","version":"1.0","type":"link"}