{"product_id":"quantichrom-indican-assay-kit-bht15600059","title":"QuantiChrom™ Indican Assay Kit","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eFor quantitative determination of indican (indoxyl sulfate) in urine samples. The assay uses OD480nm for signal readout. Compatible sample input includes Urine. Typical stated assay timing is 10 min.\u003c\/p\u003e\n\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003e\n\u003cstrong\u003eReadout format:\u003c\/strong\u003e OD480nm supports plate-based signal acquisition and consistent comparison across matched samples.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eSample compatibility:\u003c\/strong\u003e The stated sample scope includes Urine, which is useful when aligning matrix type with calibration and control design.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eAnalytical range context:\u003c\/strong\u003e The supplied specifications include a stated detection limit of 0.2 mg\/dL for interpreting low-signal samples.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eFeature emphasis:\u003c\/strong\u003e Fast and sensitive. Linear detection range: 0.2 – 20 mg\/dL (8-800 µmoles\/L).\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eAdditional feature notes highlight Fast and high-throughput. Homogeneous “mix-incubate-measure” type assay. The assay takes only 10 min. Can be readily automated for processing thousands of samples per day. Available format information for this listing includes 1000 Tests.\u003c\/p\u003e\n\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eThis product is centered on measurement of indican within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.\u003c\/p\u003e\n\n\u003ch2\u003eMore details\u003c\/h2\u003e\n\u003cp\u003e\u003ci\u003e Indican \u003c\/i\u003e(indoxyl sulfate) is a putrefaction product resulting from bacterial deconjugation of dietary tryptophan to indole in the small intestine. A large body of evidence supports the use of urinary indican testing as a beneficial point-of-care screening tool for dysbiosis, small intestinal bacterial overgrowth (SIBO), constipation, malabsorption, intestinal mucosal permeability etc. Common microorganisms that contribute to positive indican tests include\u003ci\u003eSalmonella\u003c\/i\u003e, Shigella,\u003ci\u003eCampylobacter jejuni\u003c\/i\u003e,\u003ci\u003eyersinia enterocolitica\u003c\/i\u003e,\u003ci\u003eCitrobacter freundi\u003c\/i\u003e,\u003ci\u003eCitrobacter diversus\u003c\/i\u003e, Klebsiella pneumoniae, Pseudomonas aeruginosa, certain strains of\u003ci\u003eEscherichia coli\u003c\/i\u003e,\u003ci\u003eStaphyloccocus aureus\u003c\/i\u003e, some strains of bacteriodes,\u003ci\u003eClostridium difficile\u003c\/i\u003e,\u003ci\u003eCandida albicans\u003c\/i\u003e, other candida species etc. Traditional Indican test using Obermayer’s reagent gives only qualitative results. BioAssay Systems’ quantitative indican assay kit is based on an improved Curzon and Walsh method. In this assay, indican reacts with chromogen. The color intensity of the product at 480nm is directly proportional to the indican concentration in the sample.\u003c\/p\u003e\n\n\u003ch2\u003eDetection method\u003c\/h2\u003e\n\u003cp\u003eColorimetric (OD 480 nm).\u003c\/p\u003e\n\n\u003ch2\u003eDetection limit and analytical sensitivity\u003c\/h2\u003e\n\u003cp\u003eReported detection limit: 0.2 mg\/dL.\u003c\/p\u003e\n\n\u003ch2\u003eProcedures and timing\u003c\/h2\u003e\n\u003cp\u003eStated procedure or timing information: 10 min.\u003c\/p\u003e\n\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003ePlate-based quantification and side-by-side group comparison remain central use cases for this assay format.\u003c\/li\u003e\n  \u003cli\u003eThe product notes emphasize multi-sample throughput, making it relevant for screening-oriented and larger batch comparison studies.\u003c\/li\u003e\n  \u003cli\u003eShort assay timing and plate compatibility support time-course or repeated-measure collection plans when handling is kept consistent.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eQuantify indican in urine by OD480 nm readout.\u003c\/li\u003e\n  \u003cli\u003eCompare treatment or phenotype groups using matched urine handling.\u003c\/li\u003e\n  \u003cli\u003eMonitor time-course or pre\/post changes in urine across study conditions.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eInterpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.\u003c\/p\u003e\n\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eMatrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.\u003c\/li\u003e\n  \u003cli\u003eUse appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c!-- Sources (internal):\n- Product Description column\n- Key Features column\n- More Details column\n- Method \/ Sample Type(s) \/ Assay Time \/ Detection Limit \/ Detection Method columns\n- Procedures column\n- Screening Services column\n--\u003e","brand":"BioAssay Systems","offers":[{"title":"1000 Tests","offer_id":53238315811181,"sku":"DIDC-01K","price":489.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/DIDCfig_ddf68ddd-f411-4a4e-9fbe-8a646be1da3c.jpg?v=1776668352","url":"https:\/\/www.ebiohippo.com\/products\/quantichrom-indican-assay-kit-bht15600059","provider":"BioHippo","version":"1.0","type":"link"}