{"product_id":"quantichrom-pyrophosphatase-inhibitor-assay-kit-bht15600086","title":"QuantiChrom™ Pyrophosphatase Inhibitor Assay Kit","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eFor high-throughput inhibitor screening and evaluation of pyrophosphatase modulators. The assay uses OD620 for signal readout. Compatible sample input includes Compounds that affect pyrophosphatase activity. Typical stated assay timing is 70 min.\u003c\/p\u003e\n\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003e\n\u003cstrong\u003eReadout format:\u003c\/strong\u003e OD620 supports plate-based signal acquisition and consistent comparison across matched samples.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eSample compatibility:\u003c\/strong\u003e The stated sample scope includes Compounds that affect pyrophosphatase activity, which is useful when aligning matrix type with calibration and control design.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eWorkflow timing:\u003c\/strong\u003e The listed assay time of 70 min helps frame batch planning, replicate handling, and plate throughput.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eFeature emphasis:\u003c\/strong\u003e Safe. Non-radioactive assay.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eAdditional feature notes highlight Fast and convenient. The procedure involves incubation of enzyme and inhibitor, addition of a single working reagent and incubation for 30 min. Room temperature assay. No 37°C incubator is needed; High-throughput. Homogenous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling systems. Robust assay with a Z’ factor of 0.59. Can be used in 96-well, 384-well, and potentially higher density screening assays. Available format information for this listing includes 100 Tests in 96-well plate (Custom bulk sizes available upon request).\u003c\/p\u003e\n\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eThis product is centered on measurement of pyrophosphatase inhibitor within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.\u003c\/p\u003e\n\n\u003ch2\u003eMore details\u003c\/h2\u003e\n\u003cp\u003e\u003ci\u003eINORGANIC PYROPHOSPHATASE\u003c\/i\u003e(E.C.3.6.1.1) catalyzes the hydrolysis of phosphoester bonds of inorganic pyrophosphate [P\u003csub\u003e2\u003c\/sub\u003eO\u003csub\u003e7\u003c\/sub\u003e\u003csup\u003e4-\u003c\/sup\u003e], thereby releasing two orthophosphate molecules. Family I PPases are essential enzymes found in all kingdoms of life and are responsible for maintaining the correct pyrophosphate equilibrium necessary to carry out nucleic acid and protein synthesis, and facilitate fatty acid beta oxidation.\u003c\/p\u003e\n\n\u003ch2\u003eDetection method\u003c\/h2\u003e\n\u003cp\u003eColorimetric (OD 620 nm).\u003c\/p\u003e\n\n\u003ch2\u003eProcedures and timing\u003c\/h2\u003e\n\u003cp\u003eStated procedure or timing information: 70 min.\u003c\/p\u003e\n\n\u003ch2\u003eScreening services\u003c\/h2\u003e\n\u003cp\u003eBioAssay Systems offers comprehensive Pyrophosphatase lead discovery services, including compound library screening, inhibitor profiling, and high-throughput assays to identify potential drug candidates. We provide detailed analyses of compound activity, potency, and selectivity, supporting hit-to-lead and lead optimization efforts. Please contact us by Service .\u003c\/p\u003e\n\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003ePlate-based quantification and side-by-side group comparison remain central use cases for this assay format.\u003c\/li\u003e\n  \u003cli\u003eThe product notes emphasize multi-sample throughput, making it relevant for screening-oriented and larger batch comparison studies.\u003c\/li\u003e\n  \u003cli\u003eThe description supports intervention-focused study designs in which researchers compare baseline and perturbed conditions.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eQuantify pyrophosphatase inhibitor in compounds that affect pyrophosphatase by OD620 readout.\u003c\/li\u003e\n  \u003cli\u003eCompare treatment or phenotype groups using matched compounds that affect pyrophosphatase handling.\u003c\/li\u003e\n  \u003cli\u003eMonitor time-course or pre\/post changes in compounds that affect pyrophosphatase across study conditions.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eInterpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.\u003c\/p\u003e\n\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eMatrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.\u003c\/li\u003e\n  \u003cli\u003eUse appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c!-- Sources (internal):\n- Product Description column\n- Key Features column\n- More Details column\n- Method \/ Sample Type(s) \/ Assay Time \/ Detection Limit \/ Detection Method columns\n- Procedures column\n- Screening Services column\n--\u003e","brand":"BioAssay Systems","offers":[{"title":"100 Tests in 96-well plate (Custom bulk sizes available upon request)","offer_id":53238314172781,"sku":"DPPI-100","price":410.97,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/DPPIfig.jpg?v=1776668350","url":"https:\/\/www.ebiohippo.com\/products\/quantichrom-pyrophosphatase-inhibitor-assay-kit-bht15600086","provider":"BioHippo","version":"1.0","type":"link"}