{"product_id":"quantichrom-total-carbohydrate-assay-kit-bht15600258","title":"QuantiChrom™ Total Carbohydrate Assay Kit","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eFor quantitative determination of total carbohydrate in food, beverage, biological samples (e.g. serum, plasma, etc). The assay uses OD490nm for signal readout. Compatible sample input includes Food, beverage, biological samples (e.g. serum, plasma, etc).. Typical stated assay timing is 15 min.\u003c\/p\u003e\n\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003e\n\u003cstrong\u003eReadout format:\u003c\/strong\u003e OD490nm supports plate-based signal acquisition and consistent comparison across matched samples.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eSample compatibility:\u003c\/strong\u003e The stated sample scope includes Food, beverage, biological samples (e.g. serum, plasma, etc)., which is useful when aligning matrix type with calibration and control design.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eAnalytical range context:\u003c\/strong\u003e The supplied specifications include a stated detection limit of 0.02 mM for interpreting low-signal samples.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eFeature emphasis:\u003c\/strong\u003e Non-radioactive assay.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eAdditional feature notes highlight Sensitive and accurate. Linear detection range in 96-well plate: 0.02 to 5 mM glucose equivalents for colorimetric assays; Fast and convenient. The procedure involves 15 minutes of incubation time and minimal sample preparation. Available format information for this listing includes 100 Tests in 96-well plate.\u003c\/p\u003e\n\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eThis product is centered on measurement of total carbohydrate within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.\u003c\/p\u003e\n\n\u003ch2\u003eMore details\u003c\/h2\u003e\n\u003cp\u003e\u003ci\u003eCARBOHYDRATES\u003c\/i\u003eare made up of carbon, hydrogen, and oxygen. A carbohydrate may exist in its simplest form, a monosaccharide, or as larger polymers called polysaccharides (e.g. starch, glycogen, and cellulose). Carbohydrates are essential constituents of living things, and in the form of glucose, serve as the main source of energy for metabolism. They are also commonly found in many fruits and vegetables as simple or complex sugars.BioAssay Systems’ QuantiChrom™ Total Carbohydrate Assay Kit first hydrolyzes polysaccharides to monosaccharides, which are then converted to furfural compounds that react with the Detection Reagent. The color intensity of the chromagen, measured at 490 nm, is directly proportional to the total carbohydrate concentration (glucose equivalents) in the sample.\u003c\/p\u003e\n\n\u003ch2\u003eDetection method\u003c\/h2\u003e\n\u003cp\u003eColorimetric (OD 490 nm).\u003c\/p\u003e\n\n\u003ch2\u003eDetection limit and analytical sensitivity\u003c\/h2\u003e\n\u003cp\u003eReported detection limit: 0.02 mM.\u003c\/p\u003e\n\n\u003ch2\u003eProcedures and timing\u003c\/h2\u003e\n\u003cp\u003eStated procedure or timing information: 15 min.\u003c\/p\u003e\n\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003ePlate-based quantification and side-by-side group comparison remain central use cases for this assay format.\u003c\/li\u003e\n  \u003cli\u003eShort assay timing and plate compatibility support time-course or repeated-measure collection plans when handling is kept consistent.\u003c\/li\u003e\n  \u003cli\u003eMatched standards, blanks, and replicate wells are typically used to improve interpretability across batches and sample matrices.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eQuantify total carbohydrate in food, beverage, biological samples (serum by OD490 nm readout.\u003c\/li\u003e\n  \u003cli\u003eCompare treatment or phenotype groups using matched food, beverage, biological samples (serum handling.\u003c\/li\u003e\n  \u003cli\u003eMonitor time-course or pre\/post changes in food, beverage, biological samples (serum across study conditions.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eInterpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.\u003c\/p\u003e\n\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eMatrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.\u003c\/li\u003e\n  \u003cli\u003eUse appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c!-- Sources (internal):\n- Product Description column\n- Key Features column\n- More Details column\n- Method \/ Sample Type(s) \/ Assay Time \/ Detection Limit \/ Detection Method columns\n- Procedures column\n- Screening Services column\n--\u003e","brand":"BioAssay Systems","offers":[{"title":"100 Tests in 96-well plate","offer_id":53238320922989,"sku":"TCRB-100","price":459.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/TCRBfig.jpg?v=1776668363","url":"https:\/\/www.ebiohippo.com\/products\/quantichrom-total-carbohydrate-assay-kit-bht15600258","provider":"BioHippo","version":"1.0","type":"link"}