{"product_id":"quantichromhydroxyproline-assay-kit-bht15600051","title":"QuantiChrom™Hydroxyproline Assay Kit","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eFor quantitative determination of hydroxyproline and collagen in biologic and cosmetic samples. The assay uses OD560nm for signal readout. Compatible sample input includes Hydroxyproline and collagen in biologic and cosmetic samples. Typical stated assay timing is Approx. 2 hrs.\u003c\/p\u003e\n\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003e\n\u003cstrong\u003eReadout format:\u003c\/strong\u003e OD560nm supports plate-based signal acquisition and consistent comparison across matched samples.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eSample compatibility:\u003c\/strong\u003e The stated sample scope includes Hydroxyproline and collagen in biologic and cosmetic samples, which is useful when aligning matrix type with calibration and control design.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eAnalytical range context:\u003c\/strong\u003e The supplied specifications include a stated detection limit of 0.5 µg\/mL for interpreting low-signal samples.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eFeature emphasis:\u003c\/strong\u003e Safe. Our hydroxyproline assay uses an improved perchlorate-free chemistry.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eAdditional feature notes highlight Sensitive and accurate. Uses 20 µL sample. Linear detection range 0.5 µg\/mL to 50 µg\/mL hydroxyproline in 96-well plate assay; Convenient. Collagen can be hydrolyzed in Eppendorf tubes, and final incubation can be performed at 37°C. Available format information for this listing includes 100 Tests.\u003c\/p\u003e\n\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eThis product is centered on measurement of hydroxyproline within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.\u003c\/p\u003e\n\n\u003ch2\u003eMore details\u003c\/h2\u003e\n\u003cp\u003e\u003ci\u003eHYDROXYPROLINE\u003c\/i\u003eis a unique modified amino acid that is found exclusively in several animal proteins, the most prevalent of which is collagen. Collagen is a key structural protein of the extracellular matrix and is common in connective tissues due to its incredible strength. Because of its role as a key structural protein in animals collagen is crucial for many medical applications, such as tissue transplantation and scaffolding for complex cell cultures. Collagen is also used in the beauty industry, as it can be supplemented to increase tissue rigidity, resulting in skin that appears more youthful. BioAssay Systems’ Hydroxyproline assay kit delivers a safe, simple, and sensitive means to quantify hydroxyproline in samples. In the first step of this procedure, hydroxyproline in the sample is oxidized to a pyrrole ring. This compound then reacts with a dye reagent to yield a pink product that can be measured at 560 nm. Hydroxyproline exists almost exclusively in collagen, so hydroxyproline content can be used as a proxy for collagen content.\u003c\/p\u003e\n\n\u003ch2\u003eDetection method\u003c\/h2\u003e\n\u003cp\u003eColorimetric (OD 560 nm).\u003c\/p\u003e\n\n\u003ch2\u003eDetection limit and analytical sensitivity\u003c\/h2\u003e\n\u003cp\u003eReported detection limit: 0.5 µg\/mL.\u003c\/p\u003e\n\n\u003ch2\u003eProcedures and timing\u003c\/h2\u003e\n\u003cp\u003eStated procedure or timing information: Approx. 2 hrs.\u003c\/p\u003e\n\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003ePlate-based quantification and side-by-side group comparison remain central use cases for this assay format.\u003c\/li\u003e\n  \u003cli\u003eShort assay timing and plate compatibility support time-course or repeated-measure collection plans when handling is kept consistent.\u003c\/li\u003e\n  \u003cli\u003eMatched standards, blanks, and replicate wells are typically used to improve interpretability across batches and sample matrices.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eQuantify hydroxyproline in hydroxyproline and collagen in biologic and by OD560 nm readout.\u003c\/li\u003e\n  \u003cli\u003eCompare treatment or phenotype groups using matched hydroxyproline and collagen in biologic and handling.\u003c\/li\u003e\n  \u003cli\u003eMonitor time-course or pre\/post changes in hydroxyproline and collagen in biologic and across study conditions.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eInterpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.\u003c\/p\u003e\n\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eMatrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.\u003c\/li\u003e\n  \u003cli\u003eUse appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c!-- Sources (internal):\n- Product Description column\n- Key Features column\n- More Details column\n- Method \/ Sample Type(s) \/ Assay Time \/ Detection Limit \/ Detection Method columns\n- Procedures column\n- Screening Services column\n--\u003e","brand":"BioAssay Systems","offers":[{"title":"100 Tests","offer_id":53238316302701,"sku":"DHYP-100","price":559.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/DHYPfig.jpg?v=1776668350","url":"https:\/\/www.ebiohippo.com\/products\/quantichromhydroxyproline-assay-kit-bht15600051","provider":"BioHippo","version":"1.0","type":"link"}