{"product_id":"quantifluo-angiotensin-converting-enzyme-1-assay-kit-bht15600232","title":"QuantiFluo™ Angiotensin-Converting Enzyme 1 Assay Kit","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eFor quantitative determination of ACE-1 activity determination in biological samples. Safe and convenient. Non-radioactive assay. “Mix-incubate-measure” type assay. No wash and reagent transfer steps are involved. Sensitive and accurate. The assay uses FL360\/485nm for signal readout. Compatible sample input includes Serum, cell or tissue lysate, and other biological samples. Typical stated assay timing is 45 min.\u003c\/p\u003e\n\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003e\n\u003cstrong\u003eReadout format:\u003c\/strong\u003e FL360\/485nm supports plate-based signal acquisition and consistent comparison across matched samples.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eSample compatibility:\u003c\/strong\u003e The stated sample scope includes Serum, cell or tissue lysate, and other biological samples, which is useful when aligning matrix type with calibration and control design.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eAnalytical range context:\u003c\/strong\u003e The supplied specifications include a stated detection limit of 0.01 U\/L for interpreting low-signal samples.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eFeature emphasis:\u003c\/strong\u003e Safe and convenient. Non-radioactive assay. “Mix-incubate-measure” type assay. No wash and reagent transfer steps are involved.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eAdditional feature notes highlight Sensitive and accurate. Linear detection range 0.01 – 5 U\/L ACE-1 in a 96-well plate assay; High-throughput. Can be readily automated to assay thousands of samples per day. Available format information for this listing includes 100 Tests in 96-well plate.\u003c\/p\u003e\n\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eThis product is centered on measurement of angiotensin-converting enzyme 1 within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.\u003c\/p\u003e\n\n\u003ch2\u003eMore details\u003c\/h2\u003e\n\u003cp\u003e\u003ci\u003eANGIOTENSIN-CONVERTING ENZYME 1, ACE-1 (EC 3.4.15.1)\u003c\/i\u003eis a critical enzyme in the renin-angiotensin-aldosterone system (RAAS), which regulates blood pressure, fluid, and electrolyte balance in the body. Found primarily in the lung endothelium and kidney epithelial cells, ACE-1 hydrolyzes angiotensin I, cleaving off the His-Leu dipeptide to produce angiotensin II. Its role in the RAAS system makes it a major target for hypertension and cardiovascular treatments.BioAssay Systems’ QACE1-100 Kit provides a convenient fluorometric method to measure ACE-1 activity in biological samples. In this assay, ACE-1 hydrolyzes the synthetic substrate, releasing the product. Then, o-phthalaldehyde (OPA) fluorescently labels the product for quantification. The increase in fluorescence at λ\u003csub\u003eex\/em\u003c\/sub\u003e= 360\/485 nm is directly proportional to enzyme activity.\u003c\/p\u003e\n\n\u003ch2\u003eDetection method\u003c\/h2\u003e\n\u003cp\u003eFluorescent (FL 360\/485 nm).\u003c\/p\u003e\n\n\u003ch2\u003eDetection limit and analytical sensitivity\u003c\/h2\u003e\n\u003cp\u003eReported detection limit: 0.01 U\/L.\u003c\/p\u003e\n\n\u003ch2\u003eProcedures and timing\u003c\/h2\u003e\n\u003cp\u003eStated procedure or timing information: 45 min.\u003c\/p\u003e\n\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003ePlate-based quantification and side-by-side group comparison remain central use cases for this assay format.\u003c\/li\u003e\n  \u003cli\u003eThe product notes emphasize multi-sample throughput, making it relevant for screening-oriented and larger batch comparison studies.\u003c\/li\u003e\n  \u003cli\u003eShort assay timing and plate compatibility support time-course or repeated-measure collection plans when handling is kept consistent.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eQuantify angiotensin-converting enzyme 1 in serum, cell or tissue lysate by FL360\/485 nm readout.\u003c\/li\u003e\n  \u003cli\u003eCompare treatment or phenotype groups using matched serum, cell or tissue lysate handling.\u003c\/li\u003e\n  \u003cli\u003eMonitor time-course or pre\/post changes in serum, cell or tissue lysate across study conditions.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eInterpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.\u003c\/p\u003e\n\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eMatrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.\u003c\/li\u003e\n  \u003cli\u003eUse appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c!-- Sources (internal):\n- Product Description column\n- Key Features column\n- More Details column\n- Method \/ Sample Type(s) \/ Assay Time \/ Detection Limit \/ Detection Method columns\n- Procedures column\n- Screening Services column\n--\u003e","brand":"BioAssay Systems","offers":[{"title":"100 Tests in 96-well plate","offer_id":53238321152365,"sku":"QACE1-100","price":429.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/QACE1fig.jpg?v=1776668362","url":"https:\/\/www.ebiohippo.com\/products\/quantifluo-angiotensin-converting-enzyme-1-assay-kit-bht15600232","provider":"BioHippo","version":"1.0","type":"link"}