{"product_id":"quantifluo-hrp-detection-reagent-bht15600238","title":"QuantiFluo™ HRP Detection Reagent","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eStabilized 10-Acetyl-3,7-dihydroxyphenoxazine (also called Ampliflu Red) liquid substrate reagent for detecting horseradish peroxidase HRP activity, e.g. in ELISA. This reagent produces a highly fluorescent (530\/585nm), pink-colored. The assay uses OD570nm, FL530\/585nm for signal readout. Compatible sample input includes Biological. Typical stated assay timing is 30 min.\u003c\/p\u003e\n\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003e\n\u003cstrong\u003eReadout format:\u003c\/strong\u003e OD570nm, FL530\/585nm supports plate-based signal acquisition and consistent comparison across matched samples.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eSample compatibility:\u003c\/strong\u003e The stated sample scope includes Biological, which is useful when aligning matrix type with calibration and control design.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eWorkflow timing:\u003c\/strong\u003e The listed assay time of 30 min helps frame batch planning, replicate handling, and plate throughput.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eFeature emphasis:\u003c\/strong\u003e For both absorbance or fluorescence detection.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eAdditional feature notes highlight Highly sensitive; Convenient and fast. Available format information for this listing includes 500 Tests.\u003c\/p\u003e\n\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eThis product is centered on measurement of hrp detection within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.\u003c\/p\u003e\n\n\u003ch2\u003eMore details\u003c\/h2\u003e\n\u003cp\u003eQuantiFluo™ Horseradish Peroxidase Reagent is a stable 10-Acetyl-3,7-dihydroxyphenoxazine (also called Ampliflu Red) liquid substrate reagent formulated for detecting horseradish peroxidase HRP activity, e.g. in ELISA. This reagent produces a highly fluorescent product (λex\/em = 530\/585 nm) when reacted with H\u003csub\u003e2\u003c\/sub\u003eO\u003csub\u003e2\u003c\/sub\u003eby a peroxidase. The fluorescent product can also be visualized under a light source (530 ± 20 nm).\u003c\/p\u003e\n\n\u003ch2\u003eDetection method\u003c\/h2\u003e\n\u003cp\u003eColorimetric (OD 570 nm) or Fluorescent (FL 530\/585 nm).\u003c\/p\u003e\n\n\u003ch2\u003eProcedures and timing\u003c\/h2\u003e\n\u003cp\u003eStated procedure or timing information: 30 min.\u003c\/p\u003e\n\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003ePlate-based quantification and side-by-side group comparison remain central use cases for this assay format.\u003c\/li\u003e\n  \u003cli\u003eShort assay timing and plate compatibility support time-course or repeated-measure collection plans when handling is kept consistent.\u003c\/li\u003e\n  \u003cli\u003eMatched standards, blanks, and replicate wells are typically used to improve interpretability across batches and sample matrices.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003ePrepare control-treated samples for paired use with compatible assay-kit plates.\u003c\/li\u003e\n  \u003cli\u003eBenchmark assay response against defined control conditions in replicate wells.\u003c\/li\u003e\n  \u003cli\u003eOptimize reagent concentration and incubation windows for plate-based comparisons.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eInterpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.\u003c\/p\u003e\n\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eMatrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.\u003c\/li\u003e\n  \u003cli\u003eUse appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c!-- Sources (internal):\n- Product Description column\n- Key Features column\n- More Details column\n- Method \/ Sample Type(s) \/ Assay Time \/ Detection Limit \/ Detection Method columns\n- Procedures column\n- Screening Services column\n--\u003e","brand":"BioAssay Systems","offers":[{"title":"500 Tests","offer_id":53238320103789,"sku":"QFHRP-25mL","price":297.67,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/BASRTfig_10127bf9-785b-4e67-8d08-bdf0c49a6f77.jpg?v=1776668362","url":"https:\/\/www.ebiohippo.com\/products\/quantifluo-hrp-detection-reagent-bht15600238","provider":"BioHippo","version":"1.0","type":"link"}