{"product_id":"qx-314-bromide-bhb21300293","title":"QX-314 bromide","description":"\u003ch3\u003eProduct details\u003c\/h3\u003e \u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eChemical name:\u003c\/strong\u003e N-(2,6-Dimethylphenylcarbamoylmethyl) triethylammonium bromide.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAlso known as:\u003c\/strong\u003e Lidocaine N-ethyl bromide\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCAS number:\u003c\/strong\u003e 21306-56-9\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMolecular formula:\u003c\/strong\u003e C16H27N2OBr.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurity:\u003c\/strong\u003e \u0026gt;99% (HPLC)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eConcentration:\u003c\/strong\u003e 1-50 mM.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eForm:\u003c\/strong\u003e Lyophilized powder.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSolubility:\u003c\/strong\u003e Water. Centrifuge all product preparations before use (10000 x g 5 min).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReconstitution:\u003c\/strong\u003e Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eShipping:\u003c\/strong\u003e Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e Various NaV Na+ channels\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSource:\u003c\/strong\u003e Synthetic\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eActivity:\u003c\/strong\u003e QX-314 bromide is an intracellular blocker of NaV channels1-4.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAlternative names:\u003c\/strong\u003e Lidocaine N-ethyl bromide\u003c\/li\u003e\n\u003c\/ul\u003e \u003ch3\u003eScientific background\u003c\/h3\u003e \u003cp\u003eThis local anesthetic is a blocker of voltage-dependent Na+ channels. QX-314 blocks all NaV channels once exposed to the intracellular face of the channel protein. However, this compound can penetrate the cell interior once applied outside the cell. Via certain types of NaV channels, it can permeate and exert blocking activity via TTX-sensitive channels.1 Intracellular recordings from inferior olivary neurons in brain stem slice preparations demonstrated that the Na+-dependent action potential was completely blocked for approximately five minutes after impalement with a microelectrode containing 50 mM QX-314, whereas the low-threshold Ca2+ spike was still generated.2 QX-314 is widely used to block NaV channels and used as a tool in neurophysiological recordings.2-5\u003c\/p\u003e \u003cp\u003e\u003cstrong\u003eLead time:\u003c\/strong\u003e 1-2 Business Days\u003c\/p\u003e \u003cp\u003e\u003cstrong\u003eCountry of origin:\u003c\/strong\u003e Israel\/IL\u003c\/p\u003e \u003ch3\u003eApplications key\u003c\/h3\u003e \u003cp\u003eApplication key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.\u003c\/p\u003e \u003cp\u003e\u003cstrong\u003eBioassay tested:\u003c\/strong\u003e Yes\u003c\/p\u003e \u003ch3\u003eCited application(s)\u003c\/h3\u003e \u003cp\u003eElectrophysiology\u003c\/p\u003e","brand":"Alomone Labs","offers":[{"title":"1 g \/ 1","offer_id":53249712718189,"sku":"Q-100-1G-1","price":569.0,"currency_code":"USD","in_stock":true},{"title":"100 mg \/ 1","offer_id":53311573623149,"sku":"Q-100-100MG-1","price":81.0,"currency_code":"USD","in_stock":true},{"title":"2.5 g \/ 1","offer_id":53311573655917,"sku":"Q-100-2P5G-1","price":1141.0,"currency_code":"USD","in_stock":true},{"title":"500 mg \/ 1","offer_id":53311573688685,"sku":"Q-100-500MG-1","price":317.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/Q-100_gr.gif?v=1778544451","url":"https:\/\/www.ebiohippo.com\/products\/qx-314-bromide-bhb21300293","provider":"BioHippo","version":"1.0","type":"link"}