{"product_id":"rab8a-antibody-ras-related-protein-rab-8a-bha17135785","title":"RAB8A Antibody \/ Ras related protein Rab 8A","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eRAB8A Antibody \/ Ras related protein Rab 8A is a anti-RAB8A Rabbit antibody Recombinant Rabbit Monoclonal clone 29R94 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e RAB8A\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 29R94, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC, IF, FACS\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eRAB8A antibody recognizes Ras related protein Rab 8A, a small GTP binding protein encoded by the RAB8A gene. RAB8A belongs to the Rab family of small GTPases, which regulate vesicular transport, endocytosis, and exocytosis. This protein plays a critical role in polarized membrane trafficking, cytoskeletal dynamics, and ciliogenesis. Researchers use RAB8A antibody to explore the regulatory mechanisms underlying intracellular transport and how disruptions can lead to disease.\u003cbr\u003e\u003cbr\u003eRas related protein Rab 8A cycles between an inactive GDP bound state and an active GTP bound state, allowing it to coordinate vesicle budding, transport, and fusion events. Active RAB8A recruits specific effector proteins that drive trafficking of vesicles to the plasma membrane, supporting processes such as neurite outgrowth, epithelial polarization, and secretion. Studies using RAB8A antibody have shown its localization to recycling endosomes and the Golgi network, highlighting its role in maintaining polarized trafficking routes in diverse cell types.\u003cbr\u003e\u003cbr\u003eDisruption of RAB8A function is linked to ciliopathies and neurodegenerative conditions. RAB8A regulates ciliary membrane extension and signaling, making it critical for developmental pathways such as Hedgehog signaling. Defective Rab protein regulation has also been implicated in Parkinson disease, where altered endocytic recycling and trafficking impair neuronal health. Detection with RAB8A antibody provides valuable insights into these disease mechanisms and offers a tool for studying how vesicular trafficking contributes to pathophysiology.\u003cbr\u003e\u003cbr\u003eRAB8A antibody is applied in western blotting, immunofluorescence, and immunohistochemistry. Western blotting detects expression in a range of tissues, while immunofluorescence reveals localization to endosomal compartments and the base of primary cilia. Immunohistochemistry further shows distribution in brain, kidney, and epithelial tissues, correlating with its functional roles. Functional assays using RAB8A antibody can track changes in expression following experimental perturbations of membrane trafficking pathways.\u003cbr\u003e\u003cbr\u003eBy supplying validated RAB8A antibody reagents,\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=RAB8A - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=RAB8A - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=RAB8A - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/RAB8A - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=RAB8A+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047305372013,"sku":"FY12883","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_3854f07f-39fd-4857-8cf8-775eec27448d.jpg?v=1772019356","url":"https:\/\/www.ebiohippo.com\/products\/rab8a-antibody-ras-related-protein-rab-8a-bha17135785","provider":"BioHippo","version":"1.0","type":"link"}