{"product_id":"rabbit-anti-goat-igg-h-and-l-secondary-antibody-biotin-conjugated-bha21012522","title":"Rabbit Anti-Goat IgG (H+L) Secondary Antibody, Biotin Conjugated","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eRabbit Anti-Goat IgG (H+L) Secondary Antibody, Biotin Conjugated is a Biotin conjugated secondary antibody used for indirect detection of primary antibodies and immunoglobulins from Goat IgG (H+L). It is commonly selected for experiments where label choice and species\/isotype recognition affect signal interpretation.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eHost species:\u003c\/strong\u003e Rabbit (antibody source species).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eRecognition:\u003c\/strong\u003e Designed to recognize immunoglobulins from Goat IgG (H+L) for secondary detection.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eConjugate\/label:\u003c\/strong\u003e Biotin provides a defined detection chemistry (e.g., fluorophore, enzyme, or biotin–streptavidin systems).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eClonality:\u003c\/strong\u003e Polyclonal.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSpecificity notes (provided):\u003c\/strong\u003e This biotin conjugated antibody is specific for goat IgG and shows no cross-reactivity with human\/rat\/mouse\/rabbit IgG..\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eProduct Overview Product Name Rabbit Anti-Goat IgG (H+L) Secondary Antibody, Biotin Conjugate Synonyms Biotin-conjugated Rabbit Anti-Goat IgG; Rabbit Anti-Goat IgG Biotinylated Antibody; Biotinylated Rabbit Anti-Goat IgG Secondary Antibody; Rabbit Anti-Goat IgG Secondary Antibody, Biotin-labeled Description Rabbit Anti-Goat IgG (H+L) Secondary Antibody, Biotin Conjugate, for in sensitive immunodetection and\/or quantification of low-abundance target proteins through ELISA or IHC by using reporter-labeled biotin-binding signal amplification systems Reagent Type Biotin conjugated secondary antibody Conjugate Biotin Host Rabbit Target Species Goat Antibody Class IgG Clonality Polyclonal Immunogen Whole molecule goat IgG Purification Immunoaffinity chromatography Specificity Goat IgG specific; no cross-reactivity with human\/rat\/mouse\/rabbit IgG Form Supplied Liquid: concentrated buffered stock solution Formulation 0.5 mg biotin-conjugated secondary antibody 0.01 M PBS (PH 7.4) 50% glycerol Pack Size 0.5 ml Concentration 1 mg\/ml Application ELISA, IHC Storage At -20?C for one year from date of receipt. Avoid repeated freezing and thawing. Precautions FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR CLINICAL USE Assay Information Sample Type Human primary-antibody-probed: SDS-PAGE separated-, membrane-immobilized-proteins from cell\/tissue lysates, formalin-fixed paraffin-embedded (FFPE) tissue sections on slides Assay Type Immunoanalytical Technique In immunodetection of target protein via reporter-labeled biotin-binding detection systems Assay Purpose Protein detection\/quantification Equipment Needed WB\/Dot blot\/ELISA\/IHC instrumentation; Reporter signal detectors: X-ray film cassette; a charge-coupled device (CCD) imager; Spectrophotometer; fluorescent or electron microscope Main Advantages Specific High signal-to-noise ratio High Signal Amplification Multiple secondary antibodies can bind to a single primary antibody; Multiple reporter molecules localize to a single biotin via avidin\/streptavidin bridges Fast Fewer processing steps - no need to add a substrate; Less optimization required compared to enzymatic detection; Generates strong signals in a relatively short time span; Fluorescence can be observed ly Quantifieable Allows quantification of detected signal Easy to Use Supplied in a workable liquid format Flexible Biotin- (Strept)Avidin system can be coupled with various types of reporters (enzymes, fluorochromes, fluorophores, chromophores, etc.); One type of labeled secondary antibody can be used to recognize different types of primary antibodies of the target organism specific to a particular antigen Compatible Biotin does not interfere with catalysis or antibody binding Background Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species. The host antiserum is then purified through immunoaffinity chromatography to remove all host serum proteins, except the specific antibody of interest. Purified secondary antibodies are further solid phase adsorbed with other species serum proteins to minimize cross-reactivity in tissue or cell preparations, and are then modified with antibody fragmentation, label conjugation, etc., to generate highly specific reagents. Secondary antibodies can be conjugated to a large number of labels, including enzymes, biotin, and fluorescent dyes\/proteins. Here, the antibody provides the specificity to locate the protein of interest, and the label generates a detectable signal. The label of choice depends upon the experimental application. Biotinylated antibodies are widely used in systems where signal amplification is desired. Often 15-20 biotin moieties are coupled to a single IgG secondary antibody. Biotin binds avidin, streptavidin, or neutravidin with a high degree of affinity and specificity. In immunoassays avidin\/streptavidin-biotin binding is used as a bridge between antibodies and reporters like enzymes (HRP, AP), fluorophores, chromophores, etc. Both avidin and streptavidin are tetrameric proteins capable of binding 4 biotin groups to each molecule of avidin or streptavidin, thus amplifying the signal intensity and detection sensitivity by increasing the concentration of reporters at the antigenic site. Two main biotin-binding detection systems have been widely utilized: Avidin-Biotin Complex (ABC) and Labeled Streptavidin Biotin (LSAB) methods. In the ABC method free avidin (or streptavidin) is used as a bridge\/link between the biotinylated antibody and а biotinylated reporter molecule, resulting in three reporter molecules coupled to the biotinylated antibody. The LSAB method employs a reporter-labeled streptavidin (avidin or neutravidin can alternatively be used) to detect the bound biotinylated-secondary antibody on the tissue section, blotting membrane or ELISA plate, improving the sensitivity of detection by 8-fold. The LSAB method is used when the avidin-biotin-enzyme complex in the ABC method becomes too large to penetrate the tissue.\u003c\/p\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e \u003cp\u003eSecondary antibodies enable indirect detection by binding to the constant regions of primary antibodies. Fragment specificity matters: reagents labeled as (H+L) recognize epitopes on both heavy and light chains of the target-species immunoglobulin, while Fc- or light-chain-specific secondaries can reduce off-target signal in some multiplex or isotype-specific designs. Label choice (fluorophore, enzyme, biotin) affects sensitivity, multiplex compatibility, and how signal should be interpreted across sample types.\u003c\/p\u003e \u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e \u003cul\u003e \u003cli\u003eMultiplex imaging and spatial biology workflows increasingly rely on well-matched secondary antibodies to minimize cross-reactivity between species and isotypes.\u003c\/li\u003e \u003cli\u003eBiotin–streptavidin amplification remains common for tissue staining and low-abundance targets, with controls used to monitor endogenous biotin background where relevant.\u003c\/li\u003e \u003cli\u003eQuantitative immunoassays often combine consistent secondary reagents with standardized detection chemistries to support cross-experiment comparability.\u003c\/li\u003e \u003c\/ul\u003e \u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e supports tissue-based localization and comparative staining patterns when paired with appropriate controls.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA\/plate assays:\u003c\/strong\u003e provides scalable detection in microplate formats for binding or titer-style measurements.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e \u003cul\u003e \u003cli\u003e\n\u003cstrong\u003eCross-reactivity and background:\u003c\/strong\u003e non-target binding can arise from endogenous immunoglobulins, closely related species, or multiplexed primary antibodies. Species selection and cross-adsorption (when specified) help reduce unwanted signal.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eControl concepts:\u003c\/strong\u003e no-primary controls, isotype controls, and orthogonal detection channels help distinguish specific staining from background. For biotin-based systems, consider controls for endogenous biotin where relevant.\u003c\/li\u003e \u003c\/ul\u003e \u003c!-- Sources (internal): - Understanding secondary antibodies – selection guide — Abcam — https:\/\/docs.abcam.com\/pdf\/secondary-antibodies\/secondary-antibodies-selection-guide.pdf - Choosing a secondary antibody: fragment specificity — Thermo Fisher Scientific — https:\/\/documents.thermofisher.com\/TFS-Assets\/LSG\/brochures\/TR0059-Choose-secondary-Ab.pdf - Cross-adsorbed secondary antibodies and cross-reactivity — Jackson ImmunoResearch — https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/technical-tips\/cross-adsorbed-and-cross-reactivity\/ - What does (H+L) mean? — Biotium — https:\/\/biotium.com\/faqs\/what-does-hl-mean\/ - Comparison of antibody IgG-binding proteins — Thermo Fisher Scientific — https:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/antibodies\/antibodies-learning-center\/antibodies-resource-library\/antibody-methods\/comparison-antibody-igg-binding-proteins.html - Avidin–biotin complex method for IHC detection — Thermo Fisher Scientific — https:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/protein-biology\/protein-biology-learning-center\/protein-biology-resource-library\/pierce-protein-methods\/avidin-biotin-complex-method-ihc-detection.html --\u003e","brand":"Boster Bio","offers":[{"title":"0.5 ml","offer_id":53072631234925,"sku":"BA1006-0.5ml","price":45.0,"currency_code":"USD","in_stock":true},{"title":"1 ml","offer_id":53072633954669,"sku":"BA1006-1ml","price":80.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/www.ebiohippo.com\/products\/rabbit-anti-goat-igg-h-and-l-secondary-antibody-biotin-conjugated-bha21012522","provider":"BioHippo","version":"1.0","type":"link"}