{"product_id":"rabbit-gfap-glial-fibrillary-acidic-protein-elisa-kit-bhe15212995","title":"Rabbit GFAP(Glial Fibrillary Acidic Protein) ELISA Kit","description":"\u003ch3\u003eScientific background\u003c\/h3\u003e\u003cp\u003e\u003cstrong\u003eGFAP (Glial Fibrillary Acidic Protein)\u003c\/strong\u003e is a neuroscience-related marker used to study neuronal\/glial biology, synaptic function, or neuroinflammatory processes (target dependent).\u003c\/p\u003e\u003cp\u003eChanges in marker concentrations can help relate functional outcomes (behavior, electrophysiology) to molecular changes in specific brain regions or fluids.\u003c\/p\u003e\u003cp\u003eQuantitative profiling is often most informative when combined with region-matched sampling and appropriate normalization\/controls.\u003c\/p\u003e\u003ch3\u003eWhy it matters\u003c\/h3\u003e\u003cul\u003e\n\u003cli\u003eQuantify \u003cstrong\u003eGFAP (Glial Fibrillary Acidic Protein)\u003c\/strong\u003e to compare biological changes across conditions, doses, or time points.\u003c\/li\u003e\n\u003cli\u003eGenerate concentration data from a standard curve to support biomarker and mechanistic studies.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch3\u003eHow the ELISA works\u003c\/h3\u003e\u003cp\u003eDesigned for \u003cstrong\u003eRabbit\u003c\/strong\u003e samples, this kit uses a \u003cstrong\u003eThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit GFAP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit GFAP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit GFAP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit GFAP in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/strong\u003e. After binding and washing, signal is converted to concentration using a standard curve.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSample types\u003c\/strong\u003e: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eDetection range\u003c\/strong\u003e: 62.5-4000 pg\/mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSensitivity\/LoD\u003c\/strong\u003e: 24.9 pg\/mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAssay time\u003c\/strong\u003e: 3.5h\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"ELK Biotechnology","offers":[{"title":"96 T","offer_id":52966474056045,"sku":"ELK11409-96T","price":595.4,"currency_code":"USD","in_stock":true},{"title":"48 T","offer_id":52966474088813,"sku":"ELK11409-48T","price":416.0,"currency_code":"USD","in_stock":true},{"title":"96 T X 5","offer_id":52966474121581,"sku":"ELK11409-96TX5","price":2531.1,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/1h1qbq4v21p4717oo1b_ccc30c87-9877-4512-88fc-820df476c8c1.jpg?v=1769437112","url":"https:\/\/www.ebiohippo.com\/products\/rabbit-gfap-glial-fibrillary-acidic-protein-elisa-kit-bhe15212995","provider":"BioHippo","version":"1.0","type":"link"}