{"product_id":"rad54-minus-minus-knockout-nalm-6-msh2-positive-cell-line-10-bhc10923254","title":"RAD54\u003csup\u003e-\/-\u003c\/sup\u003e Knockout Nalm-6 MSH2+ Cell Line (#10)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThe RAD54−\/− Knockout Nalm-6 MSH2⁺ Cell Line (#10) is a genetically engineered human pre-B cell line derived from the Nalm-6 background, featuring a biallelic knockout of the RAD54 gene using targeted homologous recombination (non-CRISPR method). This knockout line retains functional MSH2, allowing for the study of homologous recombination repair pathways in the context of intact mismatch repair.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e RAD54\u003csup\u003e-\/-\u003c\/sup\u003e Knockout Nalm-6 MSH2+ Cell Line (#10) is supplied as an engineered cell line derived from Human peripheral blood.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Suspension, lymphocyte-like\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Eagle's Minimal Essential Medium (EMEM) (TM511) + 10% FBS (Regular*) + 1X Non-Essential Amino Acids (TM068) + 1mM Sodium Pyruvate (TM057) + 0.15 µM Vitamin B12 + 50 µM 2-Mercaptoethanol (CH045) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. 1.0 mg\/ml Geneticin\/G418 (G271) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. *Do not heat-inactivate\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in immunology, hematology, and signaling studies. Donor\/background information is available for contextual interpretation.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eThis model supports studies in immunology, hematology, and signaling. It can be used to examine morphology, growth behavior, and experimental responses in cultured cells. Donor\/background information provided for this product: Male, 19, Lymphoblastic leukemia.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eEngineered cell lines are widely used for reporter-based readouts, perturbation studies, and assay optimization in reproducible culture systems.\u003c\/li\u003e\n\u003cli\u003eReporter or transgene-bearing models are often compared with matched parental or control cells to interpret signal changes in context.\u003c\/li\u003e\n\u003cli\u003eExpression trends are typically evaluated alongside passage number, selection pressure, and baseline growth behavior.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eRoutine expansion and maintenance of a defined cell model for downstream in vitro experiments.\u003c\/li\u003e\n\u003cli\u003ePhenotype, signaling, or marker-expression studies performed under standardized culture conditions.\u003c\/li\u003e\n\u003cli\u003eCell-based assay development in which passage number, growth surface, and medium composition are tracked as experimental variables.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Eagle's Minimal Essential Medium (EMEM) (TM511) + 10% FBS (Regular*) + 1X Non-Essential Amino Acids (TM068) + 1mM Sodium Pyruvate (TM057) + 0.15 µM Vitamin B12 + 50 µM 2-Mercaptoethanol (CH045) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. 1.0 mg\/ml Geneticin\/G418 (G271) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. *Do not heat-inactivate\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSplit Ratio:\u003c\/strong\u003e 1:6-1:10\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180552642925,"sku":"T6075","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/mYNqn489PHUwWYozMgKhoGUqbP7mCzA2b4kIBeQi.png?v=1774957887","url":"https:\/\/www.ebiohippo.com\/products\/rad54-minus-minus-knockout-nalm-6-msh2-positive-cell-line-10-bhc10923254","provider":"BioHippo","version":"1.0","type":"link"}