{"product_id":"rad54l2-antibody-rad54-like-2-bha17135932","title":"RAD54L2 Antibody \/ RAD54-like 2","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eRAD54L2 Antibody \/ RAD54-like 2 is a anti-RAD54L2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Speckled nuclear.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e RAD54L2\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC, IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eRAD54L2 antibody detects DNA repair and recombination protein RAD54-like 2, a member of the SNF2 family of ATP-dependent chromatin remodelers involved in DNA repair, replication, and transcriptional regulation. The UniProt recommended name is DNA repair and recombination protein RAD54-like 2 (RAD54L2). Also known as ARIP4, this nuclear protein interacts with DNA and chromatin-associated factors to maintain genomic stability and regulate gene expression.\u003cbr\u003e\u003cbr\u003eFunctionally, RAD54L2 antibody identifies a 1330-amino-acid protein characterized by helicase-like ATPase domains and DNA-binding motifs. RAD54L2 participates in homologous recombination repair, facilitating the remodeling of chromatin to enable access of repair enzymes to damaged DNA. It also interacts with transcriptional co-regulators and nuclear hormone receptors, integrating DNA repair with transcriptional control. Through ATP hydrolysis, RAD54L2 alters nucleosome structure, promoting chromatin relaxation at sites of DNA damage.\u003cbr\u003e\u003cbr\u003eThe RAD54L2 gene is located on chromosome 3p21.31 and is widely expressed in proliferative tissues such as testis, ovary, and thymus. RAD54L2 shares structural homology with RAD54L, another member of the SWI2\/SNF2 family involved in recombination repair, but exhibits broader functional diversity. RAD54L2 binds both double-stranded DNA and repair intermediates, enhancing strand invasion and exchange during homologous recombination. This role ensures error-free repair of double-strand breaks and prevents chromosomal rearrangements.\u003cbr\u003e\u003cbr\u003eBeyond its DNA repair function, RAD54L2 acts as a transcriptional co-regulator by binding to androgen receptor (AR) and other nuclear receptors, modulating gene transcription in response to hormonal signaling. It is also implicated in chromatin condensation and mitotic progression. Dysregulation of RAD54L2 expression has been associated with genomic instability, altered hormone signaling, and cancer development. Elevated expression has been observed in certain tumors, where it may support DNA repair efficiency and resistance to genotoxic stress.\u003cbr\u003e\u003cbr\u003eRAD54L2 antibody is widely used in DNA repair, chromatin remodeling, and cancer research. It is suitable for western blotting, immunoprecipitation, and immunofluorescence to detect RAD54L2 protein localization and expression. This antibody supports studies of homologous recombination, transcriptional regulation, and genome stability. In oncology, RAD54L2 detection aids in evaluating repair pathway activation and therapeutic response to DNA-damaging agents.\u003cbr\u003e\u003cbr\u003eStructurally, RAD54L2 contains conserved ATP-binding Walker A and B motifs and DNA-binding helicase domains characteristic of SWI2\/SNF2 family proteins. Its activity is regulated by post-translational modifications and interactions with transcriptional complexes.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=RAD54L2 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=RAD54L2 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=RAD54L2 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/RAD54L2 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=RAD54L2+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047309631853,"sku":"FY13030","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_598857f4-92dd-4bda-8825-3ca3201a5ea2.jpg?v=1782237074","url":"https:\/\/www.ebiohippo.com\/products\/rad54l2-antibody-rad54-like-2-bha17135932","provider":"BioHippo","version":"1.0","type":"link"}