{"product_id":"radicicol-bhb21300313","title":"Radicicol","description":"\u003ch3\u003eProduct details\u003c\/h3\u003e \u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eChemical name:\u003c\/strong\u003e (1aR,2Z,4E,14R,15aR)-8-Chloro-1a,14,15,15atetrahydro-9 ,11-dihydroxy-14-methyl-6H-oxireno[e][2]benzoxacyclotetradecin-6,12(7H)-dione.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAlso known as:\u003c\/strong\u003e Monorden\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCAS number:\u003c\/strong\u003e 12772-57-5\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMolecular formula:\u003c\/strong\u003e C18H17ClO6.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurity:\u003c\/strong\u003e \u0026gt;99%\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eConcentration:\u003c\/strong\u003e 1-100 nM.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eForm:\u003c\/strong\u003e Lyophilized powder.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSolubility:\u003c\/strong\u003e DMSO, ethanol. Centrifuge all product preparations before use (10000 x g 5 min).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReconstitution:\u003c\/strong\u003e Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eShipping:\u003c\/strong\u003e Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSource:\u003c\/strong\u003e Natural\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eActivity:\u003c\/strong\u003e Radicicol is an antifungal antibiotic originally discovered to be a tyrosine kinase inhibitor1. The specific inhibition of the chaperone Hsp90 confers anticancer properties to Radicicol2,3. In addition, it also exhibits anti-angiogenic activity in vivo, and reduces the expression of VEGF4,5.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAlternative names:\u003c\/strong\u003e Monorden\u003c\/li\u003e\n\u003c\/ul\u003e \u003ch3\u003eScientific background\u003c\/h3\u003e \u003cp\u003eAn antifungal macrocyclic antibiotic, originally recognized as a protein tyrosine kinase inhibitor. Radicicol inhibits p60v-src kinase activity, and tyrosine phosphorylation of p53\/56lyn.1,2 The specific inhibition of the chaperone Hsp90 confers anticancer properties to Radicicol.3,4 In eukaryotic cells, Hsp90 catalyzes the final activation step of many regulatory proteins. Cells that lose Hsp90 function are severely compromised and cannot progress through the cell cycle. Radicicol, similarly to Geldanamycin, binds to the nucleotide-binding pocket, and inhibits ATPase activity of Hsp90.5 This causes destabilization of Hsp90 client proteins (v-Src, Raf-1, ErbB2 Ras), many of which are essential for tumor cell growth.6 Oncogene induced transformation of NIH 3T3 cells (src, ras, and mos) is suppressed by radicicol and it also disrupts the K-Ras-activated signaling pathways by selective depletion of Raf kinase.7,8 In addition, it also exhibits anti-angiogenic activity in vivo, and reduces the expression of VEGF.9,10 Radicicol inhibits AP-1-, NF-kB- and serum response factor (SRF)-mediated transcription.11 It inhibits the expression of COX-2 without affecting COX-1 expression in LPS-stimulated macrophages,1 and induces the differentiation of HL-60 cells into macrophages, by blocking the cell cycle at G1 and G2 sites.12\u003c\/p\u003e \u003cp\u003e\u003cstrong\u003eLead time:\u003c\/strong\u003e 1-2 Business Days\u003c\/p\u003e \u003cp\u003e\u003cstrong\u003eCountry of origin:\u003c\/strong\u003e Israel\/IL\u003c\/p\u003e \u003ch3\u003eApplications key\u003c\/h3\u003e \u003cp\u003eApplication key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.\u003c\/p\u003e \u003cp\u003e\u003cstrong\u003eBioassay tested:\u003c\/strong\u003e Yes\u003c\/p\u003e","brand":"Alomone Labs","offers":[{"title":"0.5 mg \/ 1","offer_id":53249714192749,"sku":"R-370-0P5MG-1","price":129.0,"currency_code":"USD","in_stock":true},{"title":"1 mg \/ 1","offer_id":53311575425389,"sku":"R-370-1MG-1","price":167.0,"currency_code":"USD","in_stock":true},{"title":"1 mg \/ 5","offer_id":53311575458157,"sku":"R-370-1MG-5","price":655.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/R-370_str.gif?v=1778544450","url":"https:\/\/www.ebiohippo.com\/products\/radicicol-bhb21300313","provider":"BioHippo","version":"1.0","type":"link"}