{"product_id":"rai1-antibody-retinoic-acid-induced-protein-1-bha17135570","title":"RAI1 Antibody \/ Retinoic acid-induced protein 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eRAI1 Antibody \/ Retinoic acid-induced protein 1 is a anti-RAI1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e RAI1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC\/IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eRAI1 antibody detects Retinoic acid-induced protein 1, a transcriptional regulator implicated in neurodevelopment, circadian rhythm control, and chromatin remodeling. RAI1 functions as a dosage-sensitive transcription factor that modulates neuronal gene expression and behavior. The RAI1 antibody is widely used in genetics, neurobiology, and developmental studies to explore transcriptional regulation and syndromic neurological disorders.\u003cbr\u003e\u003cbr\u003eRAI1 is encoded by the RAI1 gene located on human chromosome 17p11.2. The protein is approximately 1906 amino acids in length and localizes predominantly to the nucleus, where it binds DNA and interacts with chromatin-modifying complexes. RAI1 acts as a transcriptional coactivator that influences gene networks involved in neuronal differentiation, circadian rhythm, and energy balance. Its expression is tightly controlled during embryonic development and in brain regions such as the hypothalamus and cortex.\u003cbr\u003e\u003cbr\u003eThe RAI1 antibody detects a 220 kilodalton band by western blot and reveals nuclear staining under immunofluorescence microscopy. Haploinsufficiency or deletion of RAI1 causes Smith-Magenis syndrome, characterized by developmental delay, sleep disturbances, and behavioral abnormalities. Conversely, duplication of the RAI1 region is associated with Potocki-Lupski syndrome, illustrating the gene's sensitivity to dosage imbalance.\u003cbr\u003e\u003cbr\u003eRAI1 influences circadian rhythm through regulation of CLOCK and BMAL1 targets and modulates neuroplasticity and metabolic pathways. It may also interact with histone-modifying enzymes to alter chromatin accessibility. Dysregulation of RAI1 expression or mutation contributes to neuropsychiatric disorders including autism spectrum disorder, schizophrenia, and intellectual disability.\u003cbr\u003e\u003cbr\u003eBecause RAI1 governs transcriptional networks essential for neuronal function and rhythmic homeostasis, it serves as a vital link between chromatin dynamics and brain physiology.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=RAI1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=RAI1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=RAI1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/RAI1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=RAI1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047296459117,"sku":"FY12667","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_008c14df-ac95-48ec-8dd6-d86cf4e27d64.jpg?v=1782237035","url":"https:\/\/www.ebiohippo.com\/products\/rai1-antibody-retinoic-acid-induced-protein-1-bha17135570","provider":"BioHippo","version":"1.0","type":"link"}