{"product_id":"ralgapa1-antibody-ral-gtpase-activating-protein-subunit-alpha-1-bha17135647","title":"RALGAPA1 Antibody \/ Ral GTPase-activating protein subunit alpha-1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eRALGAPA1 Antibody \/ Ral GTPase-activating protein subunit alpha-1 is a anti-RALGAPA1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e RALGAPA1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eRALGAPA1 antibody detects Ras-related protein Ral GTPase-activating protein subunit alpha-1, a large scaffolding and regulatory protein that modulates RalA and RalB small GTPase activity. Encoded by the RALGAPA1 gene on chromosome 14q13.2, this protein forms part of the heterodimeric RalGAP complex together with RALGAPB, acting as a GTPase-activating protein (GAP) that inactivates Ral GTPases by promoting GTP hydrolysis. Through this mechanism, RALGAPA1 negatively regulates Ral-mediated signaling pathways involved in vesicle trafficking, cytoskeletal dynamics, and cell proliferation.\u003cbr\u003e\u003cbr\u003eRALGAPA1 localizes to the cytoplasm and membranes, where it integrates signals from Ras and Ral GTPase pathways. By controlling RalA and RalB activity, RALGAPA1 influences exocyst complex formation, endocytosis, and autophagy. Loss or suppression of RALGAPA1 enhances Ral GTPase activation and promotes tumorigenesis, cell motility, and invasion. In neurons, RALGAPA1 contributes to vesicular transport and synaptic regulation, while in muscle tissue it participates in trafficking of insulin-responsive vesicles. Mutations in RALGAPA1 have been associated with neurodevelopmental delay and epilepsy, underscoring its importance in neuronal signaling.\u003cbr\u003e\u003cbr\u003eThe RALGAPA1 antibody is used in signal transduction, neurobiology, and cancer research to investigate Ral GTPase regulation and membrane trafficking. Western blot analysis identifies a 190 kilodalton band corresponding to the alpha subunit of the RalGAP complex, while immunofluorescence reveals cytoplasmic and membrane-associated staining. This antibody enables detailed characterization of RALGAPA1 expression and function in signaling networks that intersect Ras and Rho GTPase pathways.\u003cbr\u003e\u003cbr\u003eMechanistically, RALGAPA1 serves as a key inhibitor of Ral-dependent pathways that link receptor tyrosine kinases to exocytosis and cytoskeletal remodeling. Its GAP activity provides spatial and temporal control over Ral signaling to prevent aberrant growth or migration. The RALGAPA1 antibody is essential for studying how deregulation of RalGAP signaling contributes to cancer progression and neurological dysfunction.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=RALGAPA1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=RALGAPA1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=RALGAPA1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/RALGAPA1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=RALGAPA1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047298556269,"sku":"FY12744","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_5cd37844-04ae-40cd-9710-704e5ac1f5b5.jpg?v=1782237047","url":"https:\/\/www.ebiohippo.com\/products\/ralgapa1-antibody-ral-gtpase-activating-protein-subunit-alpha-1-bha17135647","provider":"BioHippo","version":"1.0","type":"link"}