{"product_id":"ralgapb-antibody-ral-gtpase-activating-protein-subunit-beta-bha17135362","title":"RALGAPB Antibody \/ Ral GTPase-activating protein subunit beta","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eRALGAPB Antibody \/ Ral GTPase-activating protein subunit beta is a anti-RALGAPB Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e RALGAPB\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eRALGAPB antibody detects Ral GTPase-activating protein subunit beta, a large scaffolding component of the RalGAP complex that inactivates small GTPases RALA and RALB. The RalGAP complex consists of catalytic (RALGAPA1 or RALGAPA2) and regulatory (RALGAPB) subunits and serves as a negative regulator of Ral signaling, which is involved in membrane trafficking, cell migration, and tumorigenesis. The RALGAPB antibody is an essential reagent for exploring Ral-mediated signaling pathways and understanding their impact on oncogenic transformation and vesicular transport.\u003cbr\u003e\u003cbr\u003eRALGAPB is encoded by the RALGAPB gene located on human chromosome 20q13.33. The protein is cytoplasmic and interacts with Ral GTPases to stimulate their intrinsic GTP hydrolysis activity, thereby switching them from active (GTP-bound) to inactive (GDP-bound) states. Through this action, RALGAPB contributes to regulation of endocytosis, exocytosis, and actin cytoskeleton dynamics. It also links Ral signaling to insulin-stimulated GLUT4 vesicle trafficking in metabolic tissues.\u003cbr\u003e\u003cbr\u003eThe RALGAPB antibody is widely used to detect the 185-200 kDa protein in western blot and to visualize cytoplasmic localization in immunofluorescence. RALGAPB forms a heterodimer with RALGAPA1 or RALGAPA2, and the resulting complex controls Ral-dependent regulation of mTOR signaling, autophagy, and vesicle movement. Studies have implicated RALGAPB in insulin sensitivity, as well as in cancer progression where Ral signaling is hyperactivated. Loss or mutation of RALGAPB leads to sustained Ral activity, promoting cellular proliferation and migration.\u003cbr\u003e\u003cbr\u003eFunctional studies using this antibody have connected RALGAPB to the modulation of cell junction dynamics and receptor trafficking. It is also implicated in neuronal vesicle regulation and autophagosome maturation.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=RALGAPB - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=RALGAPB - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=RALGAPB - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/RALGAPB - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=RALGAPB+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047288004973,"sku":"FY12459","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_7de8df4e-c39e-42c8-9d73-9316718a575b.jpg?v=1782237003","url":"https:\/\/www.ebiohippo.com\/products\/ralgapb-antibody-ral-gtpase-activating-protein-subunit-beta-bha17135362","provider":"BioHippo","version":"1.0","type":"link"}