{"product_id":"rank-nf-kappa-b-reporter-lentivirus-set-bhv19400267","title":"RANK\/NFκB Reporter Lentivirus Set","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eRANK (receptor activator of NF-κB, TNFRSF11A) is a TNF receptor superfamily member essential for osteoclast formation and bone remodeling. Engagement by its ligand RANKL, expressed by osteoblasts and stromal cells, drives the differentiation and activation of osteoclasts that resorb bone. RANK signaling activates the NF-κB pathway, a master regulator of differentiation and inflammatory gene expression, so NF-κB response elements provide a quantitative readout of RANK engagement. The RANK-RANKL-osteoprotegerin axis controls skeletal homeostasis and is dysregulated in osteoporosis, bone metastasis, and inflammatory bone loss, making RANK an important target in bone and immune research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe RANK\/NFκB Reporter Lentivirus Set is a two-vial immunotherapy reporter system for studying RANK signaling and osteoclast differentiation. One vial constitutively expresses the human RANK receptor with an antibiotic selection marker, while the other carries tandem NF-κB response elements driving a dual reporter combining secreted Gaussia luciferase (GLuc) and a fluorescent protein (eGFP or mCherry). Sequential transduction and selection generate a dual-stable cell line that responds quantitatively to RANK engagement. Secreted GLuc accumulates in conditioned media for kinetic, lysis-free sampling, while the fluorescent reporter enables microscopy and flow cytometry. Applications include osteoclast differentiation assays and studies of bone remodeling and immune signaling. Supplied as high-titer, VSV-G-pseudotyped third-generation lentiviral particles purified by PEG precipitation and sucrose gradient centrifugation.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis 2-vial immunotherapy reporter system consists of a \u003cstrong\u003eVial 1 Receptor Lentivirus\u003c\/strong\u003e encoding human RANK under a constitutive promoter with antibiotic selection, and a \u003cstrong\u003eVial 2 Reporter Lentivirus\u003c\/strong\u003e encoding tandem NFAT (or NF-κB) response elements driving a dual reporter (Gaussia Luciferase + eGFP, Gaussia Luciferase + mCherry). Sequential transduction and selection generates a dual-stable effector cell line that responds quantitatively to receptor stimulation with a ratiometric fluorescent + bioluminescent readout.\u003c\/p\u003e\n\u003cp\u003eSecreted Gaussia luciferase (where included) accumulates in conditioned media, enabling kinetic sampling without cell lysis. The combined fluorescent and luminescent outputs allow parallel microscopy-based visualization and plate-reader luminometry from the same cell population — providing assay redundancy and flexibility for potency testing formats compliant with regulatory expectations for cell-based functional assays.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"Gaussia Luciferase + eGFP \/ Puromycin + Blasticidin \/ 2x10^6 per vial","offer_id":53251623616877,"sku":"TRV-0019-SET1","price":1200.0,"currency_code":"USD","in_stock":true},{"title":"Gaussia Luciferase + mCherry \/ Puromycin + Blasticidin \/ 2x10^6 per vial","offer_id":53362031231341,"sku":"TRV-0019-SET2","price":1200.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/Immunotherapy-Reporter-Set-Construct-Diagrams-1_6af4498e-8573-4753-b7d0-7bf22d7d81e2.jpg?v=1776934143","url":"https:\/\/www.ebiohippo.com\/products\/rank-nf-kappa-b-reporter-lentivirus-set-bhv19400267","provider":"BioHippo","version":"1.0","type":"link"}