{"product_id":"rapgef5-antibody-rap-guanine-nucleotide-exchange-factor-5-bha17136080","title":"RAPGEF5 Antibody \/ Rap guanine nucleotide exchange factor 5","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eRAPGEF5 Antibody \/ Rap guanine nucleotide exchange factor 5 is a anti-RAPGEF5 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e RAPGEF5\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eRAPGEF5 antibody detects Rap guanine nucleotide exchange factor 5, a signaling protein that activates Rap1 and Rap2 small GTPases to regulate cell adhesion, polarity, and differentiation. The UniProt recommended name is Rap guanine nucleotide exchange factor 5 (RAPGEF5). This exchange factor converts inactive GDP-bound Rap GTPases into active GTP-bound forms, facilitating intracellular signaling cascades that govern morphogenesis and tissue organization.\u003cbr\u003e\u003cbr\u003eFunctionally, RAPGEF5 antibody identifies a 914-amino-acid cytoplasmic protein containing Ras exchange motif (REM) and CDC25 homology domains responsible for nucleotide exchange activity. RAPGEF5 is regulated by cyclic AMP and interacts with Rap-binding scaffolds to control cell junction assembly and migration. It acts as an important mediator of epithelial integrity and neural development.\u003cbr\u003e\u003cbr\u003eThe RAPGEF5 gene is located on chromosome 7p15.1 and is expressed in brain, lung, and epithelial tissues. Through Rap GTPase activation, RAPGEF5 coordinates cell-cell adhesion, endothelial barrier function, and axon guidance during development.\u003cbr\u003e\u003cbr\u003ePathologically, altered RAPGEF5 expression has been linked to cancer progression, vascular leakage, and neurological disorders. Dysregulation may lead to impaired Rap signaling, causing aberrant cell polarity and migration. Research using RAPGEF5 antibody supports studies in GTPase signaling, development, and cell adhesion regulation.\u003cbr\u003e\u003cbr\u003eRAPGEF5 antibody is validated for western blotting, immunohistochemistry, and immunofluorescence to detect guanine nucleotide exchange factors. \u003cbr\u003e\u003cbr\u003eStructurally, Rap guanine nucleotide exchange factor 5 contains a cAMP-binding regulatory domain and catalytic regions that mediate activation of Rap GTPases. This antibody enables detailed analysis of RAPGEF5's contribution to intracellular signaling and polarity control.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=RAPGEF5 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=RAPGEF5 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=RAPGEF5 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/RAPGEF5 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=RAPGEF5+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047313989997,"sku":"FY13178","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_e3310975-ca8d-44a4-b5e1-0f4e4ea150dc.jpg?v=1782237093","url":"https:\/\/www.ebiohippo.com\/products\/rapgef5-antibody-rap-guanine-nucleotide-exchange-factor-5-bha17136080","provider":"BioHippo","version":"1.0","type":"link"}