{"product_id":"raph1-antibody-ras-associated-and-pleckstrin-homology-domains-containing-protein-1-bha17135314","title":"RAPH1 Antibody \/ Ras-associated and pleckstrin homology domains-containing protein 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eRAPH1 Antibody \/ Ras-associated and pleckstrin homology domains-containing protein 1 is a anti-RAPH1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), ELISA with listed reactivity in Human, Mouse. Reported localization: Cytoplasm.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e RAPH1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eThe RAPH1 antibody targets Ras-associated and pleckstrin homology domains-containing protein 1, a membrane-associated adaptor encoded by the RAPH1 gene. Also known as lamellipodin, this protein coordinates actin cytoskeleton remodeling, cell migration, and adhesion through interactions with Ena\/VASP and small GTPases. Ras-associated and pleckstrin homology domains-containing protein 1 acts as a link between membrane signaling and actin polymerization, facilitating lamellipodia formation at the leading edge of motile cells. The RAPH1 antibody enables specific detection of this regulatory molecule in studies of motility, morphogenesis, and cell polarity.\u003cbr\u003e\u003cbr\u003eRas-associated and pleckstrin homology domains-containing protein 1 contains Ras-association and pleckstrin homology domains that mediate binding to activated Ras and phosphoinositides, respectively. These domains anchor RAPH1 to the plasma membrane where it recruits actin regulators such as Ena\/VASP proteins to promote filament elongation. The RAPH1 antibody supports localization studies revealing how this scaffold integrates receptor-mediated signaling with actin dynamics. Its enrichment at lamellipodia and adhesion sites underscores its central role in coordinating directional migration.\u003cbr\u003e\u003cbr\u003eRAPH1 interacts with the small GTPase Rap1 to influence integrin activation and cell adhesion. By bridging signaling pathways that control cytoskeletal organization, it contributes to epithelial integrity, immune cell motility, and neuronal pathfinding. The RAPH1 antibody allows visualization of these processes, providing insight into mechanisms governing cell migration during wound healing and development. RAPH1 dysfunction can impair motility or lead to aberrant invasion in tumor cells.\u003cbr\u003e\u003cbr\u003eLamellipodin has also been implicated in cancer progression and metastasis. Its overexpression enhances actin polymerization and invasive potential in various cancers including breast and colorectal carcinoma. The RAPH1 antibody supports analysis of these oncogenic roles by enabling quantification of protein levels in tumor tissues. Additionally, RAPH1 participates in mechanotransduction pathways by translating substrate stiffness into cytoskeletal rearrangement, linking extracellular cues to motility responses.\u003cbr\u003e\u003cbr\u003eThe RAPH1 antibody performs effectively in western blotting, immunofluorescence, and immunohistochemistry, yielding distinct membrane and cytoplasmic staining consistent with lamellipodial localization.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=RAPH1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=RAPH1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=RAPH1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/RAPH1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=RAPH1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047287415149,"sku":"FY12411","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_eb41663c-83e7-4c60-9eeb-f024c7bc4d8e.jpg?v=1782237013","url":"https:\/\/www.ebiohippo.com\/products\/raph1-antibody-ras-associated-and-pleckstrin-homology-domains-containing-protein-1-bha17135314","provider":"BioHippo","version":"1.0","type":"link"}