{"product_id":"rare-dr5-reporter-lentivirus-bhv19400039","title":"RARE\/DR5 Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe retinoic acid response element (RARE) is a DNA sequence recognized by heterodimers of the retinoic acid receptor (RAR) and the retinoid X receptor (RXR). The DR5 element, consisting of direct repeats of a half-site spaced by five nucleotides, is a high-affinity RARE. In the presence of the ligand all-trans retinoic acid, RAR\/RXR heterodimers bound to DR5 recruit coactivators and activate transcription of retinoid-responsive genes. Retinoic acid signaling is essential for embryonic development, cell differentiation, and tissue homeostasis, and modulating this pathway is therapeutically relevant in cancer and dermatology, making RARE\/DR5 reporters valuable tools for studying retinoid biology.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe RARE\/DR5 Reporter Lentivirus is a transcription factor reporter system that provides a sensitive fluorescent or luminescent readout of RAR\/RXR heterodimer transcriptional activity in mammalian cells. The construct contains tandem repeats of the consensus DR5 DNA-binding element, which is activated by RAR\/RXR heterodimers in response to all-trans retinoic acid. Stable lentiviral integration, supported by a drug selection marker, enables generation of polyclonal reporter cell lines for analysis by fluorescence microscopy, flow cytometry, or luminometry. The system is applied to study the retinoic acid signaling pathway, cell differentiation, and retinoid pharmacology. Particles are purified by PEG precipitation and sucrose gradient centrifugation, supporting efficient transduction of difficult-to-transfect cells, including primary and cryopreserved cultures.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a EGFP, Firefly Luc, GFP, Luc, mCherry, Renilla Luc, RFP reporter gene under the control of tandem consensus response elements specific for the RAR signaling pathway transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Puromycin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53251623420269,"sku":"LTV-0043-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53310899323245,"sku":"LTV-0043-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Puromycin \/ 2x10^6","offer_id":53310899356013,"sku":"LTV-0043-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Blasticidin \/ 2x10^6","offer_id":53310899388781,"sku":"LTV-0043-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53310899421549,"sku":"LTV-0043-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53310899454317,"sku":"LTV-0043-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Renilla Luc \/ Blasticidin \/ 2x10^6","offer_id":53310899487085,"sku":"LTV-0043-4SIC","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/TF-Reporter-Vector-circular_70a5ecfb-54c1-4254-a0ad-1579defce3df.jpg?v=1776934330","url":"https:\/\/www.ebiohippo.com\/products\/rare-dr5-reporter-lentivirus-bhv19400039","provider":"BioHippo","version":"1.0","type":"link"}