{"product_id":"rars2-antibody-arginyl-trna-synthetase-2-bha17135518","title":"RARS2 Antibody \/ Arginyl-tRNA synthetase 2","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eRARS2 Antibody \/ Arginyl-tRNA synthetase 2 is a anti-RARS2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e RARS2\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eRARS2 antibody detects Arginyl-tRNA synthetase 2, mitochondrial, an enzyme that catalyzes the attachment of arginine to its corresponding mitochondrial tRNA during protein synthesis. RARS2 plays a critical role in maintaining mitochondrial translation and respiratory chain function. The RARS2 antibody is used in mitochondrial biology, genetics, and neurodegenerative research to study mitochondrial protein synthesis and energy metabolism.\u003cbr\u003e\u003cbr\u003eRARS2 is encoded by the RARS2 gene located on human chromosome 6q16.1. The protein is approximately 580 amino acids long and localizes to the mitochondrial matrix, where it contributes to the translation of 13 essential oxidative phosphorylation (OXPHOS) subunits encoded by mitochondrial DNA. RARS2 functions as part of the aminoacyl-tRNA synthetase family, ensuring fidelity of tRNA charging for mitochondrial translation.\u003cbr\u003e\u003cbr\u003eThe RARS2 antibody detects a 65 kilodalton protein by western blot and reveals mitochondrial localization under confocal imaging. RARS2 ensures accurate incorporation of arginine during protein synthesis, maintaining the stoichiometry of respiratory chain complexes. Loss of RARS2 activity disrupts mitochondrial protein translation, leading to decreased OXPHOS efficiency and increased oxidative stress.\u003cbr\u003e\u003cbr\u003eMutations in RARS2 cause pontocerebellar hypoplasia type 6, a neurodegenerative disorder characterized by developmental delay, seizures, and cerebellar atrophy. In cellular models, depletion of RARS2 impairs mitochondrial respiration, ATP production, and neuronal viability. Its regulation is closely tied to mitochondrial biogenesis and stress adaptation.\u003cbr\u003e\u003cbr\u003eAs an essential enzyme for mitochondrial translation and neural health, RARS2 is a valuable model for understanding mitochondrial gene expression and energy homeostasis.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=RARS2 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=RARS2 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=RARS2 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/RARS2 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=RARS2+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047293935981,"sku":"FY12615","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_d26c0f40-2378-4860-9ed8-402cd1b821b2.jpg?v=1782237033","url":"https:\/\/www.ebiohippo.com\/products\/rars2-antibody-arginyl-trna-synthetase-2-bha17135518","provider":"BioHippo","version":"1.0","type":"link"}