{"product_id":"rasal3-antibody-ras-gtpase-activating-like-protein-3-bha17136013","title":"RASAL3 Antibody \/ Ras GTPase-activating-like protein 3","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eRASAL3 Antibody \/ Ras GTPase-activating-like protein 3 is a anti-RASAL3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse. Reported localization: Cytoplasm.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e RASAL3\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eRASAL3 antibody detects Ras GTPase-activating-like protein 3, a Ras signaling regulator that acts as a negative modulator of Ras activity in immune cells. The UniProt recommended name is Ras GTPase-activating-like protein 3 (RASAL3). This protein converts active GTP-bound Ras into its inactive GDP-bound form, thereby controlling proliferation and activation signals in lymphocytes and other hematopoietic cells.\u003cbr\u003e\u003cbr\u003eFunctionally, RASAL3 antibody identifies a 944-amino-acid cytoplasmic protein containing C2 and RasGAP domains responsible for lipid membrane binding and GTPase activation. RASAL3 inhibits Ras-dependent pathways, including ERK\/MAPK signaling, limiting excessive immune cell activation and promoting homeostasis. It acts downstream of receptor stimulation to fine-tune immune responses.\u003cbr\u003e\u003cbr\u003eThe RASAL3 gene is located on chromosome 19q13.3 and is highly expressed in natural killer cells, T cells, and macrophages. It serves as a negative feedback regulator of immune activation by controlling Ras signaling intensity following antigen or cytokine stimulation. In NK cells, RASAL3 influences cytotoxic granule release and antiviral responses.\u003cbr\u003e\u003cbr\u003ePathologically, dysregulation of RASAL3 contributes to immune dysfunction and inflammation. Loss of function enhances Ras activity, promoting lymphoproliferative and autoimmune phenotypes. In cancer, RASAL3 downregulation may facilitate tumor immune evasion by disturbing immune regulation. Research using RASAL3 antibody supports investigations into Ras pathway modulation, immune signaling, and oncogenic Ras control.\u003cbr\u003e\u003cbr\u003eRASAL3 antibody is validated for western blotting, immunohistochemistry, and flow cytometry to detect RasGAP proteins and Ras activity regulators. \u003cbr\u003e\u003cbr\u003eStructurally, Ras GTPase-activating-like protein 3 contains an N-terminal C2 lipid-binding domain and a C-terminal catalytic GAP domain. This architecture enables membrane association and inactivation of Ras. This antibody facilitates research on RASAL3's inhibitory role in signal transduction and immune modulation.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=RASAL3 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=RASAL3 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=RASAL3 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/RASAL3 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=RASAL3+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047311892845,"sku":"FY13111","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_20f95b73-4ee1-41ce-b803-0b38eb300b15.jpg?v=1782237094","url":"https:\/\/www.ebiohippo.com\/products\/rasal3-antibody-ras-gtpase-activating-like-protein-3-bha17136013","provider":"BioHippo","version":"1.0","type":"link"}