{"product_id":"rasef-antibody-rab45-ras-and-ef-hand-domain-containing-protein-bha17135579","title":"RASEF Antibody \/ RAB45 \/ Ras and EF-hand domain-containing protein","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eRASEF Antibody \/ RAB45 \/ Ras and EF-hand domain-containing protein is a anti-RASEF Rabbit antibody Recombinant Rabbit Monoclonal clone 30R48 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e RASEF\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 30R48, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC, IF, FACS\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eRASEF antibody detects Ras and EF hand domain containing protein, also known as RAB45, encoded by the RASEF gene. RASEF is a member of the Rab family of small GTP binding proteins with unique EF hand calcium binding motifs. This combination of domains links small GTPase activity with calcium regulated signaling, positioning RASEF as a multifunctional regulator of vesicular trafficking, cytoskeletal dynamics, and cell signaling. RASEF localizes to endosomal and cytoplasmic membranes, where it contributes to transport and signaling events.\u003cbr\u003e\u003cbr\u003eRASEF antibody is widely applied in cancer research, cell biology, and immunology. RASEF has been identified as a tumor suppressor gene in uveal melanoma, where mutations or promoter hypermethylation reduce its expression. Conversely, altered RASEF activity has been observed in lung and breast cancer, suggesting context dependent functions. By detecting RASEF, researchers can investigate its dual roles in tumor biology and cell signaling.\u003cbr\u003e\u003cbr\u003e Western blot assays detect RASEF protein in diverse tissue lysates. Immunohistochemistry maps expression in eye, lung, and immune tissues, while immunofluorescence highlights subcellular localization to endosomes and membranes. These methods provide robust tools for analyzing RASEF biology.\u003cbr\u003e\u003cbr\u003eRASEF contributes to immune signaling through regulation of endosomal trafficking and receptor recycling. It may influence T cell activation, antigen presentation, and cytokine signaling by modulating endosomal compartments. Its calcium binding EF hand motifs suggest integration of calcium signaling with vesicle dynamics. By applying RASEF antibody, scientists can examine how small GTPases coordinate trafficking and signaling across biological systems.\u003cbr\u003e\u003cbr\u003eBeyond immunity and cancer, RASEF has roles in ocular biology, where it is expressed in retinal tissues and linked to eye disease. It also contributes to cellular homeostasis through regulation of vesicle fusion and cytoskeletal remodeling. These diverse functions make RASEF antibody valuable for both basic science and translational research.\u003cbr\u003e\u003cbr\u003eRASEF antibody from\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=RASEF - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=RASEF - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=RASEF - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/RASEF - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=RASEF+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047297016173,"sku":"FY12676","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_f339cfd0-e2aa-450a-8fd7-99d34fc4e90c.jpg?v=1772019327","url":"https:\/\/www.ebiohippo.com\/products\/rasef-antibody-rab45-ras-and-ef-hand-domain-containing-protein-bha17135579","provider":"BioHippo","version":"1.0","type":"link"}