{"product_id":"rassf8-antibody-ras-association-domain-containing-protein-8-bha17136159","title":"RASSF8 Antibody \/ Ras association domain-containing protein 8","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eRASSF8 Antibody \/ Ras association domain-containing protein 8 is a anti-RASSF8 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse. Reported localization: Cytoplasm, Mitochondria.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e RASSF8\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC, IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eRASSF8 antibody detects Ras association domain-containing protein 8, a cytoplasmic and membrane-associated protein involved in cell adhesion, cytoskeletal organization, and tumor suppression. The UniProt recommended name is Ras association domain-containing protein 8 (RASSF8). This member of the Ras association domain family links small GTPase signaling to cytoskeletal remodeling and intercellular junction stability.\u003cbr\u003e\u003cbr\u003eFunctionally, RASSF8 antibody identifies a 413-amino-acid protein containing an RA (Ras association) domain and a coiled-coil region that mediates homo- and hetero-oligomerization. RASSF8 localizes to adherens junctions and the plasma membrane, where it interacts with proteins such as E-cadherin, beta-catenin, and SCRIB. Through these interactions, RASSF8 supports cell polarity and epithelial integrity by regulating actin cytoskeleton organization. It may also participate in Ras-related signaling pathways that influence apoptosis and cell migration.\u003cbr\u003e\u003cbr\u003eThe RASSF8 gene is located on chromosome 12p12.1 and is expressed in epithelial and endothelial tissues, with high expression in lung, colon, and skin. It acts downstream of Ras and Rap1A, functioning as a molecular adaptor to relay signals from membrane GTPases to cytoskeletal and junctional complexes. RASSF8 expression is dynamically regulated during epithelial differentiation and stress responses.\u003cbr\u003e\u003cbr\u003ePathologically, loss or reduced expression of RASSF8 is associated with various cancers, including lung, colorectal, and breast carcinoma. Its downregulation disrupts adherens junctions, promotes epithelial-mesenchymal transition (EMT), and enhances tumor cell invasion. RASSF8 may act as a tumor suppressor by maintaining cell adhesion and suppressing uncontrolled proliferation. Research using RASSF8 antibody supports studies in cancer biology, cell adhesion, and epithelial polarity regulation.\u003cbr\u003e\u003cbr\u003eRASSF8 antibody is validated for western blotting, immunohistochemistry, and immunofluorescence to detect cytoskeletal and adhesion-related proteins. \u003cbr\u003e\u003cbr\u003eStructurally, Ras association domain-containing protein 8 contains an RA domain that binds small GTPases and a C-terminal coiled-coil domain involved in multimerization and membrane targeting. This architecture allows RASSF8 to integrate Ras-related signaling with cytoskeletal remodeling. This antibody facilitates research into RASSF8's role in tumor suppression, cell adhesion, and cytoskeletal integrity.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=RASSF8 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=RASSF8 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=RASSF8 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/RASSF8 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=RASSF8+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047319429485,"sku":"FY13257","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_fdb7b7a0-d99a-428e-9e13-46acf085f244.jpg?v=1782237077","url":"https:\/\/www.ebiohippo.com\/products\/rassf8-antibody-ras-association-domain-containing-protein-8-bha17136159","provider":"BioHippo","version":"1.0","type":"link"}