{"product_id":"rat-baff-cd257-b-cell-activating-factor-elisa-kit-bhe15205043","title":"Rat BAFF\/CD257(B-Cell Activating Factor) ELISA Kit","description":"\u003ch3\u003eScientific background\u003c\/h3\u003e\u003cp\u003e\u003cstrong\u003eBAFF\/CD257 (B-Cell Activating Factor)\u003c\/strong\u003e is a cell-surface\/immune phenotype marker commonly used to characterize immune cell subsets or activation states.\u003c\/p\u003e\u003cp\u003eWhen soluble or shed forms are measurable (target-dependent), concentrations can reflect immune activation, receptor turnover, or tissue inflammation.\u003c\/p\u003e\u003cp\u003eThese measurements can complement flow cytometry by adding a soluble biomarker readout that is compatible with biofluids.\u003c\/p\u003e\u003ch3\u003eWhy it matters\u003c\/h3\u003e\u003cul\u003e\n\u003cli\u003eQuantify \u003cstrong\u003eBAFF\/CD257 (B-Cell Activating Factor)\u003c\/strong\u003e to compare biological changes across conditions, doses, or time points.\u003c\/li\u003e\n\u003cli\u003eGenerate concentration data from a standard curve to support biomarker and mechanistic studies.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch3\u003eHow the ELISA works\u003c\/h3\u003e\u003cp\u003eDesigned for \u003cstrong\u003eRat\u003c\/strong\u003e samples, this kit uses a \u003cstrong\u003eThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat BAFF\/CD257. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat BAFF\/CD257. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat BAFF\/CD257, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat BAFF\/CD257 in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/strong\u003e. After binding and washing, signal is converted to concentration using a standard curve.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSample types\u003c\/strong\u003e: Serum, plasma, tissue homogenates, cell lysates and other biological fluids.\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eDetection range\u003c\/strong\u003e: 78.13-5000 pg\/mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSensitivity\/LoD\u003c\/strong\u003e: 26 pg\/mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAssay time\u003c\/strong\u003e: 3.5h\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"ELK Biotechnology","offers":[{"title":"96 T","offer_id":52965513331053,"sku":"ELK6268-96T","price":595.4,"currency_code":"USD","in_stock":true},{"title":"48 T","offer_id":52965513363821,"sku":"ELK6268-48T","price":416.0,"currency_code":"USD","in_stock":true},{"title":"96 T X 5","offer_id":52965513396589,"sku":"ELK6268-96TX5","price":2531.1,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/1h1qbq4v21p4717oo1b_d692b04e-0edf-42c0-b75d-a26dcfa01e59.jpg?v=1771843818","url":"https:\/\/www.ebiohippo.com\/products\/rat-baff-cd257-b-cell-activating-factor-elisa-kit-bhe15205043","provider":"BioHippo","version":"1.0","type":"link"}