{"product_id":"rat-ccdc146-pre-designed-sirna-bhn20137955","title":"Rat Ccdc146 Pre-designed siRNA","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThis pre-designed siRNA set enables sequence-specific knockdown of the rat \u003cstrong\u003eCcdc146\u003c\/strong\u003e gene (NCBI Gene ID: 499980) in rat cell lines. Each set is supplied as HPLC-purified, chemically synthesized double-stranded RNA oligonucleotides in lyophilized form, ready for reconstitution and transfection. Two size formats are available: Set A (SI202094A, 3 siRNA sequences at 3×5 nmol) and Set B (SI202094B, 4 siRNA sequences at 4×10 nmol), providing flexibility for screening or extended knockdown studies.\u003c\/p\u003e\n\u003ch2\u003eKey Elements and Design Rationale\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003e\n\u003cstrong\u003eMultiple independent sequences:\u003c\/strong\u003e Both sets include 3 or 4 distinct siRNA duplexes targeting different regions of the rat \u003cstrong\u003eCcdc146\u003c\/strong\u003e mRNA, increasing the probability of achieving effective knockdown and providing built-in sequence redundancy for experimental confidence.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eGAPDH positive control:\u003c\/strong\u003e Included in every set to verify transfection efficiency and confirm that the RNAi machinery is functionally active in the cell model used.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eScrambled negative control (si-NC):\u003c\/strong\u003e A non-targeting scrambled duplex is included to control for non-specific effects of the transfection reagent and dsRNA delivery.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eFAM-labeled negative control:\u003c\/strong\u003e Fluorescently labeled NC allows direct visualization of transfection efficiency by fluorescence microscopy or flow cytometry prior to mRNA or protein readout.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eHPLC purification:\u003c\/strong\u003e Each duplex is HPLC-purified to remove truncated sequences and reagent impurities that could contribute to off-target effects.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eBiological Background\u003c\/h2\u003e\n\u003cp\u003eThe rat \u003cstrong\u003eCcdc146\u003c\/strong\u003e gene (Gene ID: 499980) encodes a protein involved in cellular function in \u003cem\u003eRattus norvegicus\u003c\/em\u003e. siRNA-mediated knockdown of \u003cstrong\u003eCcdc146\u003c\/strong\u003e in rat cell line models is commonly applied to assess the contribution of this gene to cellular processes including cell signaling, metabolism, and gene regulation. The RNA interference (RNAi) pathway exploits the RISC complex: after cellular entry, the sense (passenger) strand is degraded, and the antisense (guide) strand directs RISC-mediated cleavage of complementary \u003cstrong\u003eCcdc146\u003c\/strong\u003e mRNA, leading to mRNA degradation and reduced protein expression.\u003c\/p\u003e\n\u003ch2\u003eResearch Relevance and Current Trends\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003e\n\u003cstrong\u003eRat cell line models:\u003c\/strong\u003e siRNA knockdown of \u003cstrong\u003eCcdc146\u003c\/strong\u003e in rat cell lines enables loss-of-function studies that complement rat in vivo models, providing a faster and cost-effective approach for initial functional characterization. Rat models are widely used in cardiovascular, neuroscience, and metabolic disease research.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003ePathway validation:\u003c\/strong\u003e After identifying candidate genes in rat transcriptomic or proteomic studies, researchers commonly use siRNA knockdown to confirm the functional role of a target like \u003cstrong\u003eCcdc146\u003c\/strong\u003e before advancing to stable KO approaches.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eTarget prioritization:\u003c\/strong\u003e The availability of multiple non-overlapping siRNA sequences per set supports orthogonal knockdown confirmation, reducing false-positive conclusions in gene function studies.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon Research Applications\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003e\n\u003cstrong\u003emRNA knockdown validation by RT-qPCR:\u003c\/strong\u003e Following transfection, mRNA levels of \u003cstrong\u003eCcdc146\u003c\/strong\u003e are typically measured at 24–72 h post-transfection using RT-qPCR, with knockdown efficiency calculated relative to a rat reference gene (e.g., Actb or Gapdh).\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eProtein-level knockdown by Western blot:\u003c\/strong\u003e Knockdown at the protein level is assessed 48–96 h post-transfection. Note that mRNA and protein knockdown kinetics may differ depending on protein turnover rate.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003ePhenotypic assays:\u003c\/strong\u003e Once knockdown is confirmed, researchers use the optimized siRNA sequence to assess effects on cell viability, proliferation, migration, apoptosis, or signaling pathway activation in rat cell models.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eFAM-NC-guided optimization:\u003c\/strong\u003e The FAM-labeled negative control enables parallel assessment of transfection efficiency (≥80% is typically required) before committing target-specific siRNA to experiments.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eNotes for Experimental Interpretation\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003e\n\u003cstrong\u003eSpecies specificity:\u003c\/strong\u003e These siRNA sequences are designed for rat \u003cstrong\u003eCcdc146\u003c\/strong\u003e mRNA. They are not expected to achieve equivalent knockdown in human or mouse cell lines due to coding sequence divergence between species.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eTransfection efficiency:\u003c\/strong\u003e The FAM-labeled NC should be used first to optimize reagent and siRNA concentrations for the specific rat cell line used.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eOff-target effects:\u003c\/strong\u003e Inclusion of the scrambled negative control and comparison of multiple independent Ccdc146 siRNA sequences helps distinguish on-target from off-target phenotypes.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003emRNA vs. protein knockdown:\u003c\/strong\u003e Both mRNA (RT-qPCR) and protein (WB) readouts are recommended for complete characterization.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003ch3\u003eSet A (SI202094A) — 3 siRNA sequences × 5 nmol\u003c\/h3\u003e\n\u003cul\u003e\n  \u003cli\u003eRat Ccdc146 siRNA-1: 5 nmol (HPLC)\u003c\/li\u003e\n  \u003cli\u003eRat Ccdc146 siRNA-2: 5 nmol (HPLC)\u003c\/li\u003e\n  \u003cli\u003eRat Ccdc146 siRNA-3: 5 nmol (HPLC)\u003c\/li\u003e\n  \u003cli\u003eGAPDH siRNA Positive Control: 2.5 nmol (HPLC)\u003c\/li\u003e\n  \u003cli\u003esiRNA Negative Control: 2.5 nmol (HPLC)\u003c\/li\u003e\n  \u003cli\u003eFAM-labeled siRNA Negative Control: 2.5 nmol (HPLC)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eSet B (SI202094B) — 4 siRNA sequences × 10 nmol\u003c\/h3\u003e\n\u003cul\u003e\n  \u003cli\u003eRat Ccdc146 siRNA-1: 10 nmol (HPLC)\u003c\/li\u003e\n  \u003cli\u003eRat Ccdc146 siRNA-2: 10 nmol (HPLC)\u003c\/li\u003e\n  \u003cli\u003eRat Ccdc146 siRNA-3: 10 nmol (HPLC)\u003c\/li\u003e\n  \u003cli\u003eRat Ccdc146 siRNA-4: 10 nmol (HPLC)\u003c\/li\u003e\n  \u003cli\u003eGAPDH siRNA Positive Control: 2.5 nmol (HPLC)\u003c\/li\u003e\n  \u003cli\u003esiRNA Negative Control: 2.5 nmol (HPLC)\u003c\/li\u003e\n  \u003cli\u003eFAM-labeled siRNA Negative Control: 2.5 nmol (HPLC)\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003c!-- Sources (internal):\n- NCBI Gene: https:\/\/www.ncbi.nlm.nih.gov\/gene\/499980\n- Elbashir SM et al. (2001) Nature. https:\/\/doi.org\/10.1038\/35078107\n- Fire A et al. (1998) Nature. https:\/\/doi.org\/10.1038\/35888\n- Reynolds A et al. (2004) Nature Biotechnology. https:\/\/doi.org\/10.1038\/nbt936\n- Jackson AL \u0026 Linsley PS (2010) Nature Reviews Drug Discovery. https:\/\/doi.org\/10.1038\/nrd3054\n- Kaelin WG (2012) Science. https:\/\/doi.org\/10.1126\/science.1225150\n--\u003e","brand":"GenCefe Biotech","offers":[{"title":"3 × packageA","offer_id":53171356598637,"sku":"SI202094A","price":279.0,"currency_code":"USD","in_stock":true},{"title":"4 × packageB","offer_id":53171448316269,"sku":"SI202094B","price":399.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/pre-designed_siRNA_A-3_vials_1f29f764-aa60-45bb-b56f-c9625aa518cb.png?v=1774859533","url":"https:\/\/www.ebiohippo.com\/products\/rat-ccdc146-pre-designed-sirna-bhn20137955","provider":"BioHippo","version":"1.0","type":"link"}