{"product_id":"rat-cntf-protein-bhp13700328","title":"Rat CNTF Protein","description":"\u003ch2\u003eBackground\u003c\/h2\u003e\u003cp\u003e\u003cstrong\u003eCNTF\u003c\/strong\u003e is supplied as a recombinant protein reagent for \u003cstrong\u003eresearch use only\u003c\/strong\u003e. In RUO settings, recombinant proteins provide defined inputs for biochemical assays, interaction mapping, and assay development where control over protein identity and concentration supports reproducibility.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAlso known as:\u003c\/strong\u003e Ciliary neurotrophic factor; CNTF; H-CNTF.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSpecies origin:\u003c\/strong\u003e Rat.\u003c\/p\u003e\u003cp\u003eCiliary neurotrophic factor (CNTF) is a member of the cytokine family. It is a polypeptide hormone that has the function of promoting the synthesis of neurotransmitters and the growth of neurites in a specific neuronal population. Its action seems to be limited to the nervous system. Ciliary neurotrophic factor (CNTF) exerts its biological effects by activating a multi-subunit receptor complex consisting of an extracellular CNTF-binding subunit (CNTFα) and two transmembrane signaling proteins (glycoprotein gp130 and LIF receptor). CNTF is thought to be a potent survival factor in neurons and oligodendrocytes and may be associated with reduced tissue destruction during inflammatory episodes. CNTF is also a survival factor for peripheral sensory sympathetic ganglions and ciliary ganglia neurons.\u003c\/p\u003e\u003cp\u003eEndotoxin: \u0026lt;1 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\u003ch2\u003eBiological significance and function\u003c\/h2\u003e\u003cp\u003e\u003cstrong\u003eCNTF\u003c\/strong\u003e is used in RUO research to interrogate molecular mechanisms, interaction networks, and pathway-linked phenotypes in experimental systems. This target is frequently investigated in research themes such as \u003cstrong\u003eMolecular \u0026amp; Cellular Biology\u003c\/strong\u003e.\u003c\/p\u003e\u003ch2\u003eMolecular characteristics\u003c\/h2\u003e\u003cp\u003e\u003cstrong\u003eMolecular characteristics:\u003c\/strong\u003e Protein domains, oligomeric state, and modification-sensitive surfaces can influence binding behavior and functional readouts in vitro. Where relevant, isoforms and PTMs may alter activity, stability, or interaction specificity.\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eSource species:\u003c\/strong\u003e Rat\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMolecular weight:\u003c\/strong\u003e 22.9 kDa\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProtein length:\u003c\/strong\u003e The recombinant Rat CNTF consisting of 200 amino acids and has a calculated molecular mass of 22.9 kDa.It migrates as an approximately 24 kDa band in SDS-PAGE under reducing conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpression region:\u003c\/strong\u003e Amino acid sequence derived from the processed form of Rat-CNTF (P20294)(Met 1-Met 200) was expressed\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurity:\u003c\/strong\u003e \u0026gt; 97 % as determined by SDS-PAGE\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBiological activity:\u003c\/strong\u003e Measured by its ability to induce proliferation in TF-1 cells. The ED50 for this effect is 0.02748 ng\/mL.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003ePost-translational considerations:\u003c\/strong\u003e E. coli expression typically yields a non-glycosylated recombinant form. This is often suitable for many intracellular enzymes and binding studies, while PTM-dependent targets may show differences when glycosylation or specific disulfide-bond patterns are required.\u003c\/p\u003e\u003ch2\u003eExpression and purification strategy\u003c\/h2\u003e\u003cp\u003e\u003cstrong\u003eExpression system:\u003c\/strong\u003e E. coli. Expression system selection can influence folding state and PTM profile, which may affect binding or activity for PTM-sensitive targets.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eTagging:\u003c\/strong\u003e No tag tags are commonly used to streamline purification and enable capture\/immobilization in interaction assays. Tag presence or removal can influence some binding measurements depending on assay design.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eFormulation:\u003c\/strong\u003e Liquid in from sterile 20 mM Tris 50mM NaCl ,pH 8.0. Formulation and buffer composition can influence stability, aggregation propensity, and assay background in downstream biochemical experiments.\u003c\/p\u003e\u003ch2\u003eResearch interpretation\u003c\/h2\u003e\u003cp\u003e\u003cstrong\u003eResearch interpretation:\u003c\/strong\u003e Recombinant protein reagents enable controlled experiments such as interaction reconstitution, quantitative calibration, and mechanistic perturbation with defined inputs. Interpretation is strengthened by pairing the primary readout with orthogonal markers that report on pathway state, localization, and complex assembly.\u003c\/p\u003e","brand":"Abbkine Scientific Co., Ltd.","offers":[{"title":"5 ug","offer_id":52997764481389,"sku":"PRP1222-5UG","price":69.0,"currency_code":"USD","in_stock":true},{"title":"20 ug","offer_id":52997764514157,"sku":"PRP1222-20UG","price":189.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":52997764546925,"sku":"PRP1222-100UG","price":569.0,"currency_code":"USD","in_stock":true},{"title":"1 mg","offer_id":52997764579693,"sku":"PRP1222-1MG","price":2369.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/www.ebiohippo.com\/products\/rat-cntf-protein-bhp13700328","provider":"BioHippo","version":"1.0","type":"link"}