{"product_id":"rat-hs-crp-high-sensitivity-c-reactive-protein-microsample-elisa-kit-bhe15212681","title":"Rat hs-CRP(High Sensitivity C Reactive Protein) Microsample ELISA Kit","description":"\u003ch3\u003eScientific background\u003c\/h3\u003e\u003cp\u003e\u003cstrong\u003ehs-CRP(High Sensitivity C Reactive Protein) Microsample\u003c\/strong\u003e is an acute-phase inflammatory marker that can rise during systemic inflammation, infection, or tissue injury.\u003c\/p\u003e\u003cp\u003eAcute-phase proteins are often used to monitor overall inflammatory burden and compare disease severity across cohorts.\u003c\/p\u003e\u003cp\u003eBecause they integrate signals from multiple inflammatory pathways, they are useful for screening and for tracking response-to-treatment trends over time.\u003c\/p\u003e\u003ch3\u003eWhy it matters\u003c\/h3\u003e\u003cul\u003e\n\u003cli\u003eQuantify \u003cstrong\u003ehs-CRP(High Sensitivity C Reactive Protein) Microsample\u003c\/strong\u003e to compare biological changes across conditions, doses, or time points.\u003c\/li\u003e\n\u003cli\u003eGenerate concentration data from a standard curve to support biomarker and mechanistic studies.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch3\u003eHow the ELISA works\u003c\/h3\u003e\u003cp\u003eDesigned for \u003cstrong\u003eRat\u003c\/strong\u003e samples, this kit uses a \u003cstrong\u003eThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat hs-CRP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat hs-CRP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat hs-CRP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat hs-CRP in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/strong\u003e. After binding and washing, signal is converted to concentration using a standard curve.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSample types\u003c\/strong\u003e: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eDetection range\u003c\/strong\u003e: 1.56-100 µg\/mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSensitivity\/LoD\u003c\/strong\u003e: 1.08 µg\/mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAssay time\u003c\/strong\u003e: 3.5h\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"ELK Biotechnology","offers":[{"title":"96 T","offer_id":52966442893677,"sku":"ELK10185MS-96T","price":488.8,"currency_code":"USD","in_stock":true},{"title":"48 T","offer_id":52966442926445,"sku":"ELK10185MS-48T","price":344.5,"currency_code":"USD","in_stock":true},{"title":"96 T X 5","offer_id":52966442959213,"sku":"ELK10185MS-96TX5","price":2077.4,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/1h1qbq4v21p4717oo1b_41fd73bf-4465-47fc-bcb7-8ba514b4ffab.jpg?v=1769437117","url":"https:\/\/www.ebiohippo.com\/products\/rat-hs-crp-high-sensitivity-c-reactive-protein-microsample-elisa-kit-bhe15212681","provider":"BioHippo","version":"1.0","type":"link"}