{"product_id":"rat-il-2sra-cd25-soluble-interleukin-2-receptor-alpha-chain-microsample-elisa-kit-bhe15211238","title":"Rat IL-2sRa\/CD25(Soluble Interleukin-2 Receptor alpha chain) Microsample ELISA Kit","description":"\u003ch3\u003eScientific background\u003c\/h3\u003e\u003cp\u003e\u003cstrong\u003eIL-2sRa\/CD25(Soluble Interleukin-2 Receptor alpha chain) Microsample\u003c\/strong\u003e is an interleukin-family cytokine that helps coordinate immune cell communication and inflammatory responses.\u003c\/p\u003e\u003cp\u003eInterleukin levels often shift quickly after immune stimulation, making them useful for time-course studies of inflammation and immune activation.\u003c\/p\u003e\u003cp\u003eQuantifying interleukins can help interpret pathway engagement alongside phenotypic readouts (e.g., immune cell infiltration, fever, or tissue injury markers).\u003c\/p\u003e\u003ch3\u003eWhy it matters\u003c\/h3\u003e\u003cul\u003e\n\u003cli\u003eQuantify \u003cstrong\u003eIL-2sRa\/CD25(Soluble Interleukin-2 Receptor alpha chain) Microsample\u003c\/strong\u003e to compare biological changes across conditions, doses, or time points.\u003c\/li\u003e\n\u003cli\u003eGenerate concentration data from a standard curve to support biomarker and mechanistic studies.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch3\u003eHow the ELISA works\u003c\/h3\u003e\u003cp\u003eDesigned for \u003cstrong\u003eRat\u003c\/strong\u003e samples, this kit uses a \u003cstrong\u003eThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat IL-2sRa\/CD25. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat IL-2sRa\/CD25. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat IL-2sRa\/CD25, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat IL-2sRa\/CD25 in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/strong\u003e. After binding and washing, signal is converted to concentration using a standard curve.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSample types\u003c\/strong\u003e: serum, plasma, tissue homogenates, cell lysates and other biological fluids.\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eDetection range\u003c\/strong\u003e: 0.16-10 ng\/mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSensitivity\/LoD\u003c\/strong\u003e: 0.06 ng\/mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAssay time\u003c\/strong\u003e: 3.5h\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"ELK Biotechnology","offers":[{"title":"96 T","offer_id":52966280855917,"sku":"ELK5694MS-96T","price":595.4,"currency_code":"USD","in_stock":true},{"title":"48 T","offer_id":52966280888685,"sku":"ELK5694MS-48T","price":416.0,"currency_code":"USD","in_stock":true},{"title":"96 T X 5","offer_id":52966280921453,"sku":"ELK5694MS-96TX5","price":2531.1,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/1h1qbq4v21p4717oo1b_24b2f0cb-d556-4fac-b1a2-ce7e22117a54.jpg?v=1771847409","url":"https:\/\/www.ebiohippo.com\/products\/rat-il-2sra-cd25-soluble-interleukin-2-receptor-alpha-chain-microsample-elisa-kit-bhe15211238","provider":"BioHippo","version":"1.0","type":"link"}