{"product_id":"rat-il1r1-interleukin-1-receptor-type-i-microsample-elisa-kit-bhe15212384","title":"Rat IL1R1(Interleukin 1 Receptor Type I) Microsample ELISA Kit","description":"\u003ch3\u003eScientific background\u003c\/h3\u003e\u003cp\u003e\u003cstrong\u003eIL1R1(Interleukin 1 Receptor Type I) Microsample\u003c\/strong\u003e is an interleukin-family cytokine that helps coordinate immune cell communication and inflammatory responses.\u003c\/p\u003e\u003cp\u003eInterleukin levels often shift quickly after immune stimulation, making them useful for time-course studies of inflammation and immune activation.\u003c\/p\u003e\u003cp\u003eQuantifying interleukins can help interpret pathway engagement alongside phenotypic readouts (e.g., immune cell infiltration, fever, or tissue injury markers).\u003c\/p\u003e\u003ch3\u003eWhy it matters\u003c\/h3\u003e\u003cul\u003e\n\u003cli\u003eQuantify \u003cstrong\u003eIL1R1(Interleukin 1 Receptor Type I) Microsample\u003c\/strong\u003e to compare biological changes across conditions, doses, or time points.\u003c\/li\u003e\n\u003cli\u003eGenerate concentration data from a standard curve to support biomarker and mechanistic studies.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch3\u003eHow the ELISA works\u003c\/h3\u003e\u003cp\u003eDesigned for \u003cstrong\u003eRat\u003c\/strong\u003e samples, this kit uses a \u003cstrong\u003eThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat IL1R1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat IL1R1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat IL1R1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat IL1R1 in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/strong\u003e. After binding and washing, signal is converted to concentration using a standard curve.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSample types\u003c\/strong\u003e: Serum, plasma, tissue homogenates and other biological fluids.\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eDetection range\u003c\/strong\u003e: 31.25-2000 pg\/mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSensitivity\/LoD\u003c\/strong\u003e: 14.9 pg\/mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAssay time\u003c\/strong\u003e: 3.5h\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"ELK Biotechnology","offers":[{"title":"96 T","offer_id":52966413369709,"sku":"ELK0640MS-96T","price":595.4,"currency_code":"USD","in_stock":true},{"title":"48 T","offer_id":52966413402477,"sku":"ELK0640MS-48T","price":416.0,"currency_code":"USD","in_stock":true},{"title":"96 T X 5","offer_id":52966413435245,"sku":"ELK0640MS-96TX5","price":2531.1,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/1h1qbq4v21p4717oo1b_c54b11e5-8b47-40c8-bc76-77e1cdce2af4.jpg?v=1771848583","url":"https:\/\/www.ebiohippo.com\/products\/rat-il1r1-interleukin-1-receptor-type-i-microsample-elisa-kit-bhe15212384","provider":"BioHippo","version":"1.0","type":"link"}