{"product_id":"rat-mesenchymal-stem-cells-negative-bone-marrow-rmsc-bm-bhc18500112","title":"Rat Mesenchymal Stem Cells - Bone Marrow (RMSC-bm)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eRat Mesenchymal Stem Cells - Bone Marrow (RMSC-bm)\u003c\/strong\u003e is a cell model used for research applications where physiologically relevant identity and donor background support interpretation of experimental readouts. Rat Mesenchymal Stem Cells derived from Bone Marrow (Bone Marrow) within the Blood system.\u003c\/p\u003e\n\u003cp\u003eMesenchymal stem cells (MSC) are a well-characterized population of adult stem cells. They have the potential to develop into mature cells that produce fat, cartilage, bone, tendons, and muscle [1, 2]. The developmental plasticity of MSC has generated tremendous interest because of the potential use of mesenchymal stem cells in regenerative medicine to replace damaged tissues. MSC cultured without serum in the presence of transformation growth factors will differentiate into chondrocytes. In contrast, MSC cultured in serum with ascorbic acid and dexamethasone will differentiate into osteoblasts. With their renewal capability, MSC have the potential to be transplanted into an injured site or seeded on a biomimetic scaffold to generate appropriate tissue constructs. iXCells Biotechnologies provides high quality Rat Mesenchymal Stem Cells – Bone Marrow (RMSC-bm), which are isolated from adult rat bone marrow and cryopreserved at P1, with \u0026gt;0.5 million cells in each vial. RMSC-bm are positive for CD29, CD73, CD90, CD105, and negative for CD34, CD45. These cells are also characterized by lipid staining after differentiation. RMSC-bm are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi and can further expand for 3-4 population Mesenchymal Stem Cell Medium (Cat# MD-0037) without losing their multipotent properties. Figure 1. Adipocyte differentiation from Rat MSC-bone marrow (oil red staining, Day 14 post adipogenic induction).\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003e\n\u003cstrong\u003eCell identity:\u003c\/strong\u003e Mesenchymal Stem Cells (Primary Cells)\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eSource context:\u003c\/strong\u003e Bone Marrow; Bone Marrow; Blood\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eDonor background:\u003c\/strong\u003e Age: Adult\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eBiosafety level:\u003c\/strong\u003e BSL-1 (follow your institution’s biosafety program and local regulations)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eProduct-specific elements (such as tissue source, donor background, and cell classification) help frame how results should be interpreted across assays and experimental conditions.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eStem and progenitor cell models are widely used to study differentiation programs, lineage commitment, and regenerative responses under controlled culture perturbations.\u003c\/p\u003e\u003cp\u003eAcross primary and specialty cell models, experimental outcomes can be influenced by donor heterogeneity, passage history, confluence, and media composition. For interpretation, it is common to validate key markers or functional phenotypes in the user’s assay context and to document culture variables consistently.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eIncreasing use of primary and specialty cells to improve translational relevance for target biology and phenotypic screening.\u003c\/li\u003e\n  \u003cli\u003eAdoption of 3D culture formats and co-culture systems to better capture tissue microenvironments and cell–cell interactions.\u003c\/li\u003e\n  \u003cli\u003eIntegration of functional readouts with single-cell and multi-omics profiling to connect phenotype with molecular state.\u003c\/li\u003e\n  \u003cli\u003eExpansion of high-dimensional immune phenotyping and perturbation screens to map activation states and functional programs.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eProfile identity markers by flow cytometry or immunostaining in cultured cells\u003c\/li\u003e\n  \u003cli\u003eInduce lineage differentiation and track marker changes over a maturation time-course\u003c\/li\u003e\n  \u003cli\u003eQuantify functional responses to defined stimuli relevant to the model system\u003c\/li\u003e\n  \u003cli\u003eCompare baseline phenotype across donors\/conditions using gene expression profiling\u003c\/li\u003e\n  \u003cli\u003eEvaluate multipotency using lineage-specific staining and gene expression panels\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eInterpretation typically focuses on how a perturbation (e.g., cytokine exposure, metabolic stress, genetic manipulation, or compound treatment) shifts marker profiles or functional readouts relative to an appropriate control matched for donor and culture variables.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eDonor-to-donor heterogeneity can influence baseline phenotype and treatment response; include biological replicates when feasible.\u003c\/li\u003e\n  \u003cli\u003ePassage number, confluence, and media composition can shift gene expression and functional readouts; track and report these variables consistently.\u003c\/li\u003e\n  \u003cli\u003eContamination control (including routine mycoplasma monitoring) supports reproducibility in downstream assays.\u003c\/li\u003e\n  \u003cli\u003eUse appropriate negative\/positive controls for the readout (e.g., unstimulated controls, pathway agonists\/antagonists) to contextualize observed changes.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c!-- Sources (internal):\n- ATCC Animal Cell Culture Guide — ATCC — https:\/\/www.atcc.org\/resources\/culture-guides\/animal-cell-culture-guide\n- Cell Line Authentication — ATCC — https:\/\/www.atcc.org\/resources\/culture-guides\/cell-line-authentication\n- Biosafety in Microbiological and Biomedical Laboratories (BMBL) — U.S. HHS\/CDC\/NIH — https:\/\/www.cdc.gov\/labs\/BMBL.html\n- Mycoplasma contamination in cell culture — NCBI Bookshelf\/PMC — https:\/\/www.ncbi.nlm.nih.gov\/pmc\/\n- Primary cell culture considerations — Nature Methods — https:\/\/www.nature.com\/nmeth\/\n- Good cell culture practice guidelines — OECD\/ECVAM (concept) — https:\/\/www.oecd.org\/\n--\u003e\n\u003cp style=\"display:none\"\u003eSKU:BHC18500112\u003c\/p\u003e","brand":"iXCells Biotechnologies","offers":[{"title":"Cryopreserved \/ 0.5 million cells\/vial","offer_id":53197811908973,"sku":"10RA-023","price":772.34,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/10RA-023.png?v=1775378649","url":"https:\/\/www.ebiohippo.com\/products\/rat-mesenchymal-stem-cells-negative-bone-marrow-rmsc-bm-bhc18500112","provider":"BioHippo","version":"1.0","type":"link"}