{"product_id":"rat-primary-bladder-microvascular-endothelial-cells-bhc10901910","title":"Rat Primary Bladder Microvascular Endothelial Cells","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eRat Primary Bladder Microvascular Endothelial Cells are isolated from rat bladder microvasculature and display characteristic cobblestone morphology along with endothelial-specific marker expression. These cells provide a relevant in vitro model for studying bladder vascular biology, angiogenesis, barrier function, inflammation, and mechanisms of urinary tract and bladder diseases. The product is supplied as frozen cells.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eBiological source:\u003c\/strong\u003e Rat (R. norvegicus)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTissue origin:\u003c\/strong\u003e Kidney\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Adherent, polygonal\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Frozen\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSeeding guidance:\u003c\/strong\u003e 6,000 - 10,000 cells\/cm²\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBiosafety level:\u003c\/strong\u003e II\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCulture context:\u003c\/strong\u003e PriCoat™ T25 Flasks (G299) coated with Gelatin Coating Solution (0.1%) (TM063) are required for optimal cell adhesion and growth. Endothelial Cell Medium Kit, Low Serum (TM155) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eEndothelial cells line the luminal surface of blood vessels and regulate barrier properties, hemostatic balance, immune cell trafficking, angiogenic signaling, and tissue-specific exchange processes.\u003c\/p\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003ePrimary endothelial models are used to study barrier integrity, inflammatory activation, and permeability changes in tissue-specific vascular beds.\u003c\/li\u003e\n\u003cli\u003eCo-culture systems with immune, stromal, or parenchymal cells are increasingly used to model microenvironment-dependent signaling.\u003c\/li\u003e\n\u003cli\u003eResearchers often compare donor, vessel-bed, or organ-specific phenotypes to capture biologically relevant heterogeneity.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAssess barrier function, adhesion molecule regulation, or cytokine-driven endothelial activation in vitro.\u003c\/li\u003e\n\u003cli\u003eModel angiogenic responses, matrix interactions, and vessel-bed-specific signaling under defined culture conditions.\u003c\/li\u003e\n\u003cli\u003eUse in co-culture or transwell systems to study leukocyte transmigration and paracrine crosstalk.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eProduct-specific data supplied for this listing\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e PriCoat™ T25 Flasks (G299) coated with Gelatin Coating Solution (0.1%) (TM063) are required for optimal cell adhesion and growth. Endothelial Cell Medium Kit, Low Serum (TM155) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSeeding Density (cells\/cm²):\u003c\/strong\u003e 6,000 - 10,000\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003ePrimary cells can show donor-dependent and passage-dependent variation, so morphology, growth rate, and marker expression should be interpreted in the context of the specific lot and culture history.\u003c\/li\u003e\n\u003cli\u003eAttachment matrix, medium formulation, and gas conditions can materially influence phenotype maintenance and experimental readouts; use the recommended culture system where possible.\u003c\/li\u003e\n\u003cli\u003eCryopreserved recovery conditions matter for viability and downstream behavior. We recommend using serum-free CryoGuard™ Freezing Media (TM078).\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp style=\"display:none\"\u003eSKU:BHC10901910\u003c\/p\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"5x10\u003csup\u003e5\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53195754045805,"sku":"T4938","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/x3wB154pW0XkoL16Cg5pbbD7UFpBV3eZ3VQoO4ie.png?v=1775322483","url":"https:\/\/www.ebiohippo.com\/products\/rat-primary-bladder-microvascular-endothelial-cells-bhc10901910","provider":"BioHippo","version":"1.0","type":"link"}