{"product_id":"rat-primary-lung-epithelial-cells-bhc10901881","title":"Rat Primary Lung Epithelial Cells","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eRat Primary Lung Epithelial Cells are isolated from healthy rat lung tissue and provide a physiologically relevant in vitro model for studying pulmonary epithelial biology, barrier function, and respiratory disease mechanisms. These cells retain characteristic epithelial morphology and marker expression, making them suitable for research in toxicology, inflammation, infection, and drug discovery. The product is supplied as frozen cells.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eBiological source:\u003c\/strong\u003e Rat (R. norvegicus)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTissue origin:\u003c\/strong\u003e Lung\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Adherent, cobble-stone\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Frozen\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSeeding guidance:\u003c\/strong\u003e 10,000 - 15,000 cells\/cm²\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBiosafety level:\u003c\/strong\u003e II\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCulture context:\u003c\/strong\u003e PriCoat™ ECM T25 Flasks (G999) or Applied Cell Extracellular Matrix (G422) are required for cell adhesion to the culture vessels. Epithelial Cell Medium Kit (TM156) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eEpithelial cells form polarized barriers that regulate transport, secretion, and defense at tissue interfaces. Their phenotype is shaped by cell polarity, junctional organization, and tissue-specific differentiation cues.\u003c\/p\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003ePrimary epithelial models are widely used to examine barrier integrity, cytokine responses, and transport processes under physiologic conditions.\u003c\/li\u003e\n\u003cli\u003eResearchers often combine epithelial cells with stromal or immune components to model tissue microenvironments more faithfully.\u003c\/li\u003e\n\u003cli\u003eDonor-matched or disease-context epithelial cells help capture inter-individual heterogeneity in response assays.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eMeasure barrier integrity, junctional organization, and polarized responses in vitro.\u003c\/li\u003e\n\u003cli\u003eEvaluate cytokine, pathogen, or drug responses in tissue-relevant epithelial cultures.\u003c\/li\u003e\n\u003cli\u003eUse in co-culture or air-liquid interface-adjacent workflows when epithelial context matters.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eProduct-specific data supplied for this listing\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e PriCoat™ ECM T25 Flasks (G999) or Applied Cell Extracellular Matrix (G422) are required for cell adhesion to the culture vessels. Epithelial Cell Medium Kit (TM156) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSeeding Density (cells\/cm²):\u003c\/strong\u003e 10,000 - 15,000\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003ePrimary cells can show donor-dependent and passage-dependent variation, so morphology, growth rate, and marker expression should be interpreted in the context of the specific lot and culture history.\u003c\/li\u003e\n\u003cli\u003eAttachment matrix, medium formulation, and gas conditions can materially influence phenotype maintenance and experimental readouts; use the recommended culture system where possible.\u003c\/li\u003e\n\u003cli\u003eCryopreserved recovery conditions matter for viability and downstream behavior. We recommend using serum-free CryoGuard™ Freezing Media (TM078).\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp style=\"display:none\"\u003eSKU:BHC10901881\u003c\/p\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"5x10\u003csup\u003e5\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53195753455981,"sku":"T4800","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/yZ7UvUlSDWpsshWFbTCyseeQC3YkN1QaM03w4jhG.png?v=1775322482","url":"https:\/\/www.ebiohippo.com\/products\/rat-primary-lung-epithelial-cells-bhc10901881","provider":"BioHippo","version":"1.0","type":"link"}